Association of the ubiquitin specific peptidase 9X -linked and Afadin expression patterns with sexual maturation in boar testis
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Closely correlated expression patterns between ubiquitin specific peptidase 9X-linked (USP9X) and adherens junction formation factor (Afadin) in mouse testis development suggests that Usp9x regulates the deubiquitination of Af-6 (also known as Afadin, AFDN), and subsequently, the cell adhesion dynamics during gametogenesis. However, this relationship has not yet been tested in other domestic animals. The study was examined the temporal and spatial expression patterns of porcine USP9X and AFDN from the pre-pubertal to adult stages using real time-PCR and immunohistochemistry. Furthermore, we detected the transcripts of USP9X and AFDN in the testis of 1-, 6- and 12-months old boar, respectively. USP9X and AFDN were found to have similar expressions patterns, with basal expression after 1 month followed by a significant up-regulation from 6 months (puberty) onwards. In addition, neither the AFDN or USP9X proteins were detected in spermatogenic cells but they were expressed in the leydig cells and sertoli cells. USP9X was detected around the basal lamina during pre-puberty, and predominantly expressed in the leydig cells at puberty. Finally, in adult testis, USP9X was increased at the sertoli cell-cell interface and the sertoli cell-spermatid interface. In summary, closely correlated expression patterns between USP9X and AFDN in boar testis supports the previous findings in mice. Furthermore, the junction connections between the sertoli cells may be regulated by the ubiquitination process mediated via USP9X.
Abstract
Closely correlated expression patterns between ubiquitin specific peptidase 9X-linked (USP9X) and adherens junction formation factor (Afadin) in mouse testis development suggests that Usp9x regulates the deubiquitination of Af-6 (also known as Afadin, AFDN), and subsequently, the cell adhesion dynamics during gametogenesis. However, this relationship has not yet been tested in other domestic animals. The study was examined the temporal and spatial expression patterns of porcine USP9X and AFDN from the pre-pubertal to adult stages using real time-PCR and immunohistochemistry. Furthermore, we detected the transcripts of USP9X and AFDN in the testis of 1-, 6- and 12-months old boar, respectively. USP9X and AFDN were found to have similar expressions patterns, with basal expression after 1 month followed by a significant up-regulation from 6 months (puberty) onwards. In addition, neither the AFDN or USP9X proteins were detected in spermatogenic cells but they were expressed in the leydig cells and sertoli cells. USP9X was detected around the basal lamina during pre-puberty, and predominantly expressed in the leydig cells at puberty. Finally, in adult testis, USP9X was increased at the sertoli cell-cell interface and the sertoli cell-spermatid interface. In summary, closely correlated expression patterns between USP9X and AFDN in boar testis supports the previous findings in mice. Furthermore, the junction connections between the sertoli cells may be regulated by the ubiquitination process mediated via USP9X.
참고문헌 (17)
- Avram Hershko, "THE UBIQUITIN SYSTEM", Annual Review of Biochemistry, 67(1), pp.425~479 (1998), Annual Reviews[DOI]
- Mark Hochstrasser, "Ubiquitin, proteasomes, and the regulation of intracellular protein degradation", Current Opinion in Cell Biology, 7(2), pp.215~223 (1995), Elsevier BV[DOI]
- Rohini Bose, "Ubiquitin–Proteasome System in Spermatogenesis", Advances in Experimental Medicine and Biology, 759, pp.181~213 (2014), Springer New York[DOI]
- Bharathi Suresh, "The role of deubiquitinating enzymes in spermatogenesis", Cellular and Molecular Life Sciences, 72(24), pp.4711~4720 (2015), Springer Science and Business Media LLC[DOI]
- Takeshi Sato, "A close correlation in the expression patterns of Af-6 and Usp9x in Sertoli and granulosa cells of mouse testis and ovary", Reproduction, 128(5), pp.583~594 (2004), Bioscientifica[DOI]
- Kasane Kishi, "Spermatogonial deubiquitinase USP9X is essential for proper spermatogenesis in mice", Reproduction, 154(2), pp.135~143 (2017), Bioscientifica[DOI]
- Shinichiro Taya, "The Ras Target AF-6 is a Substrate of the Fam Deubiquitinating Enzyme", Journal of Cell Biology, 142(4), pp.1053~1062 (1998), Rockefeller University Press[DOI]
- C. Yan Cheng, "Cell Junction Dynamics in the Testis: Sertoli-Germ Cell Interactions and Male Contraceptive Development", Physiological Reviews, 82(4), pp.825~874 (2002), American Physiological Society[DOI]
- Sun-Young Baek, "Altrenogest affects expression of galectin-3 and fibroblast growth factor 9 in the reproductive tract of sows", Animal Biotechnology, 32(5), pp.537~543 (2020), Informa UK Limited[DOI]
- X. Chen, "On the conservation of function of the Drosophila Fat facets deubiquitinating enzyme and Fam, its mouse homolog", Development Genes and Evolution, 210(12), pp.603~610 (2000), Springer Science and Business Media LLC[DOI]
- J.A. Fischer-Vize, "The fat facets gene is required for Drosophila eye and embryo development", Development, 116(4), pp.985~1000 (1992), The Company of Biologists[DOI]
- M. Jones, "The Drosophila developmental gene fat facets has a human homologue in Xp11.4 which escapes X-inactivation and has related sequences on Yq11.2", Human Molecular Genetics, 5(11), pp.1695~1701 (1996), Oxford University Press (OUP)[DOI]
- G. M. Brown, "Characterisation of the coding sequence and fine mapping of the human DFFRY gene and comparative expression analysis and mapping to the Sxrb interval of the mouse Y chromosome of the Dffry gene", Human Molecular Genetics, 7(1), pp.97~107 (1998), Oxford University Press (OUP)[DOI]
- Nicola M. Hall, "Usp9y (ubiquitin-specific protease 9 gene on the Y) is associated with a functional promoter and encodes an intact open reading frame homologous to Usp9x that is under selective constraint", Mammalian Genome, 14(7), pp.437~447 (2003), Springer Science and Business Media LLC[DOI]
- Shinichiro Taya, "The deubiquitinating enzyme Fam interacts with and stabilizes β-catenin", Genes to Cells, 4(12), pp.757~767 (1999), Wiley[DOI]
- R. D. Allrich, "Pubertal Development of the Boar: Age-Related Changes in Testicular Morphology and in Vitro Production of Testosterone and Estradiol-17β", Biology of Reproduction, 28(4), pp.902~909 (1983), Oxford University Press (OUP)[DOI]
- J. J. Ford, "Sertoli cell differentiation in pubertal boars", Journal of Animal Science, 87(8), pp.2536~2543 (2009), Oxford University Press (OUP)[DOI]