Differential Expressions of Aquaporin Subtypes in the Adult Mouse Testis
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Many efforts have been made to study the expression of aquaporins (AQP) in the mammalian reproductive system, but there are not enough data available regarding their localized expression to fully understand their specific roles in male reproduction. The present study investigated the expression and localization patterns of different AQP subtypes in the adult mouse testes and testicular spermatozoa using an immunofluorescence assay. All the studied AQPs were expressed in the testes and revealed subtype-specific patterns in the intensity and localization depending on the cell types of the testes. AQP7 was the most abundant and intensive AQP subtype in the seminiferous tubules, expressing in Leydig cells and Sertoli cells as well as all stages of germ cells, especially the spermatids and testicular spermatozoa. The expression pattern of AQP3 was similar to that of AQP7, but with higher expression in the basal and lower adluminal compartments rather than the upper adluminalcompartment. AQP8 expression was limited to the spermatogonia and Leydig cells whereas AQP9 expression was exclusive to tails of the testicular spermatozoa and elongated spermatids. Taken together, the abundance and distribution of the AQPs across the different cell types in the testes indicating to their relavance in spermatogenesis, as well as in sperm maturation, transition, and function.
Abstract
Many efforts have been made to study the expression of aquaporins (AQP) in the mammalian reproductive system, but there are not enough data available regarding their localized expression to fully understand their specific roles in male reproduction. The present study investigated the expression and localization patterns of different AQP subtypes in the adult mouse testes and testicular spermatozoa using an immunofluorescence assay. All the studied AQPs were expressed in the testes and revealed subtype-specific patterns in the intensity and localization depending on the cell types of the testes. AQP7 was the most abundant and intensive AQP subtype in the seminiferous tubules, expressing in Leydig cells and Sertoli cells as well as all stages of germ cells, especially the spermatids and testicular spermatozoa. The expression pattern of AQP3 was similar to that of AQP7, but with higher expression in the basal and lower adluminal compartments rather than the upper adluminalcompartment. AQP8 expression was limited to the spermatogonia and Leydig cells whereas AQP9 expression was exclusive to tails of the testicular spermatozoa and elongated spermatids. Taken together, the abundance and distribution of the AQPs across the different cell types in the testes indicating to their relavance in spermatogenesis, as well as in sperm maturation, transition, and function.
참고문헌 (50)
- Alves MG, "CFTR regulation of aquaporin-mediated water transport : A target in male fertility", Curr Drug Targets, 16, pp.993~1006 (2015)
- An F, "Immunohistochemical study of aquaporin 1 and 2 in the reproductive tract of the Bactrian camel(Camelus bactrianus)", Int J Morphol, 34, pp.1218~1222 (2016)
- Arena S, "Aquaporin-9immunohistochemistry in varicocele testes as a consequence of hypoxia in the sperm production site", Andrologia, 43, pp.34~37 (2011)
- Arnaoutova I, "Aquaporin 1 is important for maintaining secretory granule biogenesis in endocrine cells", Mol Endocrinol, 22, pp.1924~1934 (2008)
- Arrighi S, "Immunolocalization of aquaporin water channels in the domestic cat male genital tract", Reprod Domest Anim, 49, pp.17~26 (2014)
- Azad AK, "Human aquaporins : Functional diversity and potential roles in infectious and non-infectious diseases", Front Genet, 12, pp.654865~ (2021)
- Badran HH, "Expression and regulation of aquaporins 1, 8, and 9 in the testis, efferent ducts, and epididymis of adult rats and during postnatal development", J Androl, 23, pp.358~373 (2002)
- Bernardino RL, "Estrogen modulates glycerol permeability in sertoli cells through downregulation of aquaporin-9", Cells, 7, pp.153~ (2018)
- Boj M, "Aquaporin biology of spermatogenesis and sperm physiology in mammals and teleosts", Biol Bull, 229, pp.93~108 (2015)
- Calamita G, "Possible involvement of aquaporin-7 and-8 in rat testis development and spermatogenesis", Biochem Biophys Res Commun, 288, pp.619~625 (2001)
- Calamita G, "Expression and localization of the aquaporin-8 water channel in rat testis", Biol Reprod, 64, pp.1660~1666 (2001)
- Carrageta DF, "Aquaporins and male(in)fertility : Expression and role throughout the male reproductive tract", Arch Biochem Biophys, 679, pp.108222~ (2020)
- Chen Q, "Aquaporin3 is a sperm water channel essential for postcopulatory sperm osmoadaptation and migration", Cell Res, 21, pp.922~933 (2011)
- Danyu L, "Aquaporin 1 expression in the testis, epididymis and vas deferens of postnatal ICR mice", Cell Biol Int, 32, pp.532~541 (2008)
- Delporte C, "Aquaporins and gland secretion", Adv Exp Med Biol, 969, pp.63~79 (2017)
- Domeniconi RF, "Aquaporin 9(AQP9)localization in the adult dog testis excurrent ducts by immunohistochemistry", Anat Rec(Hoboken), 290, pp.1519~1525 (2007)
- Dubé E, "Alterations in gene expression in the caput epididymides of nonobstructive azoospermic men", Biol Reprod, 78, pp.342~351 (2008)
- Elkjær ML, "Immunolocalization of AQP9 in liver, epididymis, testis, spleen, and brain", Biochem Biophys Res Commun, 276, pp.1118~1128 (2000)
- Elkjær ML, "Immunolocalization of aquaporin-8 in rat kidney, gastrointestinal tract, testis, and airways", Am J Physiol Renal Physiol, 281, pp.F1047~F1057 (2001)
- Ford J Jr, "Ductuli efferentes of the male Golden Syrian hamster reproductive tract", Andrology, 2, pp.510~520 (2014)
- Hermo L, "Thirsty business : Cell, region, and membrane specificity of aquaporins in the testis, efferent ducts, and epididymis and factors regulating their expression", J Androl, 32, pp.565~575 (2011)
- Kageyama Y, "Expression of aquaporins 7and 8 in the developing rat testis", Andrologia, 33, pp.165~169 (2001)
- Klein C, "Region-specific expression of aquaporin subtypes in equine testis, epididymis, and ductus deferens", Anat Rec, 296, pp.1115~1126 (2013)
- Laforenza U, "Aquaporinmediated water and hydrogen peroxide transport is involved in normal human spermatozoa functioning", Int J Mol Sci, 18, pp.66~ (2017)
- Levine N, "Micropuncture studies of the electrochemical aspects of fluid and electrolyte transport in individual seminiferous tubules, the epididymis and the vas deferens in rats", J Physiol, 213, pp.557~570 (1971)
- Lu DY, "Expression and immunohistochemical localization of aquaporin-1 in male reproductive organs of the mouse", Anat Histol Embryol, 37, pp.1~8 (2008)
- Ma T, "Nephrogenic diabetes insipidus in mice lacking aquaporin-3 water channels", Proc Natl Acad Sci USA, 97, pp.4386~4391 (2000)
- Meli R, "New perspectives on the potential role of aquaporins(AQPs)in the physiology of inflammation", Front Physiol, 9, pp.101~ (2018)
- Mirabella N, "Differential abundances of AQP3 and AQP5 in reproductive tissues from dogs with and without cryptorchidism", Anim Reprod Sci, 228, pp.106735~ (2021)
- Moretti E, "Immunolocalization of aquaporin 7 in human sperm and its relationship with semen parameters", Syst Biol Reprod Med, 58, pp.129~135 (2012)
- Nicchia GP, "Tissue distribution and membrane localization of aquaporin-9 water channel : Evidence for sex-linked differences in liver", J Histochem Cytochem, 49, pp.1547~1556 (2001)
- Nihei K, "Immunolocalization of aquaporin-9 in rat hepatocytes and Leydig cells", Arch Histol Cytol, 64, pp.81~88 (2001)
- Oliveira RL, "Differential expression and seasonal variation on aquaporins 1 and 9 in the male genital system of big fruiteating bat Artibeus lituratus", Gen Comp Endocrinol, 186, pp.116~125 (2013)
- Pastor-Soler N, "Aquaporin 9 expression along the male reproductive tract", Biol Reprod, 65, pp.384~393 (2001)
- Prieto-Martínez N, "Aquaporins 7 and 11 in boar spermatozoa : Detection, localisation and relationship with sperm quality", Reprod Fertil Dev, 28, pp.663~672 (2016)
- Ribeiro JC, "Aquaporins and(in)fertility : More than just water transport", BBA-Mol Basis Dis, 1867, pp.166039~ (2021)
- Saito K, "Localization of aquaporin-7 in human testis and ejaculated sperm : Possible involvement in maintenance of sperm quality", J Urol, 172, pp.2073~2076 (2004)
- Rato L, "Tubular fluid secretion in the seminiferous epithelium : Ion transporters and aquaporins in Sertoli cells", J Membr Biol, 236, pp.215~224 (2010)
- Ribeiro JC, "Aquaporins and animal gamete cryopreservation : Advances and future challenges", Animals, 12, pp.359~ (2022)
- Shivaraj SM, "Understanding aquaporin transport system in eelgrass (Zostera marina L.), an aquatic plant species", Front Plant Sci, 3(8), pp.1334~ (2017)
- Skowronski MT, "AQP7 is localized in capillaries of adipose tissue, cardiac and striated muscle : Implications in glycerol metabolism", Am J Physiol Renal Physiol, 292, pp.F956~F965 (2007)[DOI]
- Sohara E, "Morphologic and functional analysis of sperm and testes in aquaporin 7 knockout mice", Fertil Steril, 87, pp.671~676 (2007)
- Squillacioti C, "Aquaporins are differentially regulated in canine cryptorchid efferent ductules and epididymis", Animals, 11, pp.1539~ (2021)
- Tani T, "Immunolocalization of aquaporin-8 in rat digestive organs and testis", Arch Histol Cytol, 64, pp.159~168 (2001)
- Verkman AS, "Aquaporins in clinical medicine", Annu Rev Med, 63, pp.303~316 (2012)
- Vicente-Carrillo A, "Membrane stress during thawing elicits redistribution of aquaporin 7 but not of aquaporin 9 in boar spermatozoa", Reprod Domest Anim, 51, pp.665~679 (2016)
- Yang B, "Phenotype analysis of aquaporin-8 null mice", Am J Physiol Cell Physiol, 288, pp.C1161~C1170 (2005)[DOI]
- Yeung CH, "Aquaporin isoforms involved in physiological volume regulation of murine spermatozoa", Biol Reprod, 80, pp.350~357 (2009)
- Yeung CH, "Aquaporins in the human testis and spermatozoa-identification, involvement in sperm volume regulation and clinical relevance", Int J Androl, 33, pp.629~641 (2010)
- Yu L, "GATA2regulates body water homeostasis through maintaining aquaporin 2 expression in renal collecting ducts", Mol Cell Biol, 34, pp.1929~1941 (2014)
