본문 바로가기
KERD
논문KCI 등재

Effects of lipopolysaccharide and CpG-DNA on burn-induced skin injury

김용선|김동범|최인근|박재봉|권혁일|권형주|Byoung Kwon Park|Sunhee Cho|Jae-Nam Seo|Young-Hee Lee
생화학분자생물학회 (Korean Society Of Biochemistry And Molecular Biology)|2011.04|BMB Reports|제44권 제4호|pp. 273-278

0

조회

0

다운로드

0

인용

0

참고문헌

키워드

LPSCytokinesBurnCpG-DNA
주제분류화학

초록

Destruction of the skin barrier by thermal injury induces microbial invasion, which can lead to the development of systemic infection and septic shock. Microbial pathogens possess pathogen-associated molecular patterns (PAMPs), which are recognized by conserved receptors. To understand the role of PAMPs in thermal injury-induced mice, LPS or CpG-DNA were topically applied to dorsal skin after thermal injury. We observed an increase in the number of inflammatory cell infiltrates as well as thickening in the dermis upon treatment with LPS or CpG-DNA. We also found that expression of IL-1β, MIP-2, and RANTES induced by thermal injury was enhanced by LPS or CpG-DNA. In addition, the proportions of CD4+ and CD8+ T cells in the spleen and lymph nodes were altered by LPS or CpG-DNA. These results provide important information concerning PAMPs-induced inflammation upon thermal injury and provide a basis for studying the role of PAMPs in thermal injury-induced complications. [BMB reports 2011; 44(4): 273-278]

Abstract

Destruction of the skin barrier by thermal injury induces microbial invasion, which can lead to the development of systemic infection and septic shock. Microbial pathogens possess pathogen-associated molecular patterns (PAMPs), which are recognized by conserved receptors. To understand the role of PAMPs in thermal injury-induced mice, LPS or CpG-DNA were topically applied to dorsal skin after thermal injury. We observed an increase in the number of inflammatory cell infiltrates as well as thickening in the dermis upon treatment with LPS or CpG-DNA. We also found that expression of IL-1β, MIP-2, and RANTES induced by thermal injury was enhanced by LPS or CpG-DNA. In addition, the proportions of CD4+ and CD8+ T cells in the spleen and lymph nodes were altered by LPS or CpG-DNA. These results provide important information concerning PAMPs-induced inflammation upon thermal injury and provide a basis for studying the role of PAMPs in thermal injury-induced complications. [BMB reports 2011; 44(4): 273-278]

참고문헌 (43)

  1. Teodorczyk-Injeyan, J. A, "Impairment of T cell activation in burn patients: a possible mechanism of thermal injury induced immunosuppression", Clin. Exp. Immunol, 65, pp.570~581 (1986)
  2. Moss, N. M, "Temporal correlation of impaired immune response after thermal injury with susceptibility to infection in a murine model", Surgery, 104, pp.882~887 (1988)
  3. Harris, B. H, "The immune response to trauma", Semin. Pediatr. Surg, 4, pp.77~82 (1995)
  4. O’Sullivan, S. T, "Immunosuppression following thermal injury: the pathogenesis of immunodysfunction", Br. J. Plast. Surg, 50, pp.615~623 (1997)
  5. Schwacha, M. G, "Macrophages and post-burn immune dysfunction", Burns, 29, pp.1~14 (2003)
  6. Faunce, D. E, "The effects of acute ethanol exposure on cellular immunity in a murine model of thermal injury", J. Leukoc. Biol, 62, pp.733~740 (1997)
  7. O’Riordain, M. G, "Modulation of macrophage hyperactivity improves survival in a burn-sepsis model", Arch. Surg, 127, pp.152~157 (1992)
  8. Gibran, N. S, "Monocyte chemoattractant protein-1 mRNA expression in the human burn wound", J. Surg. Res, 70, pp.1~6 (1997)
  9. Schwacha, M. G, "Thermal injury induced immunosuppression in mice: the role of macrophage derived reactive nitrogen intermediates", J. Leukoc. Biol, 63, pp.51~58 (1998)
  10. Armstrong, D. A, "Neutrophil chemoattractant genes KC and MIP-2 are expressed in different cell populations at sites of surgical injury", J. Leukoc. Biol, 75, pp.641~648 (2004)
  11. Sayeed, M. M, "Alterations in cell signaling and related effector functions in T lymphocytes in burn", trauma, Shock, pp.1996, 5[DOI]
  12. O’Sullivan, S. T, "Major injury leads to predominance of the T helper-2 lymphocyte phenotype and diminished interleukin-12 production associated with decreased resistance to infection", Ann. Surg, 222, pp.482~492 (1995)
  13. Dipietro, L. A, "Modulation of macrophage recruitment into wounds by monocyte chemoattractant protein- 1", Wound Repair Regen, 9, pp.28~33 (2001)
  14. Faunce, D. E, "Neutrophil chemokine production in the skin following scald injury", Burns, 25, pp.403~410 (1999)
  15. Shallo, H, "Monocyte chemoattractant protein-1 (MCP-1) and macrophage infiltration into the skin after burn injury in aged mice", Burns, 29, pp.641~647 (2003)
  16. Drost, A. C, "Plasma cytokines after thermal injury and their relationship to infection", Ann. Surg, 218, pp.74~78 (1993)
  17. Meakins, J. L, "Etiology of multiple organ failure", J. Trauma, 30, pp.S165~S168 (1990)
  18. Baue, A. E, "Systemic inflammatory response syndrome (SIRS), multiple organ dysfunction syndrome (MODS), multiple organ failure (MOF): are we winning the battle?", Shock, 10, pp.79~89 (1998)
  19. Huh, W. K, "Dynamic alteration of human beta-defensin 2 localization from cytoplasm to intercellular space in psoriatic skin", J. Mol. Med, 80, pp.678~684 (2002)
  20. Lehrer, R. I, "Defensins: antimicrobial and cytotoxic peptides of mammalian cells", Annu. Rev. Immunol, 11, pp.105~128 (1993)
  21. Ganz, T, "Defensins: antimicrobial peptides of innate immunity", Nat. Rev. Immunol, 3, pp.710~720 (2003)
  22. Harder, J, "Isolation and characterization of human betadefensin- 3, a novel human inducible peptide antibiotic", J. Biol. Chem, 276, pp.5707~5713 (2001)
  23. Harder, J, "A peptide antibiotic from human skin", Nature, 387, pp.861~ (1997)
  24. Valore, E. V, "Human beta-defensin- 1: an antimicrobial peptide of urogenital tissues", J. Clin. Invest, 101, pp.1633~1642 (1998)
  25. Schröder, J. M, "Epithelial peptide antibiotics", Biochem. Pharmacol, 57, pp.121~134 (1999)
  26. Schmid, P, "An intrinsic antibiotic mechanism in wounds and tissue-engineered skin", J. Invest. Dermatol, 116, pp.471~472 (2001)
  27. Butmarc, J, "Human β-defensin-2 expression is increased in chronic wounds", Wound Repair Regen, 12, pp.439~443 (2004)
  28. Singh, P. K, "Production of beta-defensins by human airway epithelia", Proc. Natl. Acad. Sci. U.S.A, 95, pp.14961~14966 (1998)
  29. Hiratsuka, T, "Identification of human beta-defensin-2 in respiratory tract and plasma and its increase in bacterial pneumonia", Biochem. Biophys. Res. Commun, 249, pp.943~947 (1998)
  30. Ashitani, J, "Plasma and BAL fluid concentrations of antimicrobial peptides in patients with Mycobacterium avium-intracellulare infection", Chest, 119, pp.1131~1137 (2001)
  31. Aderem, A, "Toll-like receptors in the induction of the innate immune response", Nature, 406, pp.782~787 (2000)
  32. Anderson, K. V, "Toll signaling pathways in the innate immune response", Curr. Opin. Immunol, 12, pp.13~19 (2000)
  33. Akira, S, "Pathogen recognition and innate immunity", Cell, 124, pp.783~801 (2006)
  34. Deng, G. M, "Intra-articularly localized bacterial DNA containing CpG motifs induces arthritis", Nat. Med, 5, pp.702~705 (1999)
  35. Sparwasser, T, "Immunostimulatory CpG-oligodeoxynucleotides cause extramedullary murine hemopoiesis", J. Immunol, 162, pp.2368~2374 (1999)
  36. Heikenwalder, M, "Lymphoid follicle destruction and immunosuppression after repeated CpG oligodeoxynucleotide administration", Nat. Med, 10, pp.187~192 (2004)
  37. Kim, D, "Immunostimulation and anti-DNA antibody production by backbone modified CpG-DNA", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 379, pp.362~367 (2009), Elsivier Ltd
  38. Byoung Kwon Park, "Protection of burn-induced skin injuries by the flavonoid kaempferol", BMB reports, 43(1), pp.46~51 (2010), 한국생화학분자생물학회
  39. 김동범, "Regulation of macrophage inflammatory protein-2 geneexpression in response to 2,4-dinitrofluorobenzene in RAW264.7 cells", BMB reports, 41(4), pp.316~321 (2008), 한국생화학분자생물학회
  40. Kwon, H. J, "Regulation of macrophage inflammatory protein-2 gene expression in response to oligodeoxynucleotide containing CpG motifs in RAW 264.7 cells", Biochem. Biophys. Res. Commun, 308, pp.608~613 (2003)
  41. Lee, K. W, "CG sequence- and phosphorothioate backbone modification-dependent activation of the NF-κB-responsive gene expression by CpG- oligodeoxynucleotides in human RPMI 8226 B cells", MOLECULAR IMMUNOLOGY, 41, pp.955~964 (2004), PERGAMON-ELSEVIER SCIENCE LTD
  42. Lee, K. W, "Immunostimulatory oligodeoxynucleotide isolated from genome wide screening of Mycobacterium bovis chromosomal DNA", MOLECULAR IMMUNOLOGY, 43, pp.2107~2118 (2006), PERGAMON-ELSEVIER SCIENCE LTD
  43. Walker, H. L, "A standard animal burn", J. Trauma, 8, pp.1049~1051 (1968)

관련 논문AI추천

Serial Changes of Heat Shock Protein 70 and Interleukin-8 in Burn Blister Fluid윤현, 곽인숙, 김도헌, 김혜원, 서강열, 서동국, 유기철, 조용세, 최기훈대한피부과학회 · Annals of Dermatology2017 · 제29권 제2호Changes in the Levels of Interleukins 6, 8, and 10, Tumor Necrosis Factor Alpha, and Granulocyte-colony Stimulating Factor in Korean Burn Patients: Relation to Burn Size and Postburn Time김도헌, 김종현, 김현수, 임해준대한진단검사의학회 · Annals of Laboratory Medicine2012 · 제32권 제5호Adjuvant effect of liposome-encapsulated natural phosphodiester CpG-DNA권상훈, 권혁일, 권형주, 김동범, 박진서, 최수영, Chi-Seok Ahn, Younghee Lee생화학분자생물학회 · BMB Reports2011 · 제44권 제11호Activation of Toll-like receptor 9 and production of epitope specific antibody by liposome-encapsulated CpG-DNA권형주, 김동범, 이영희생화학분자생물학회 · BMB Reports2011 · 제44권 제9호The production and immunostimulatory activity of double-stranded CpG-DNA권형주, 김동범, 박진서, 이영희, 최수영, Byoung Kwon Park, Hyun-Jeong Seok, Jae Won Rhee, Min-Soo Kim생화학분자생물학회 · BMB Reports2010 · 제43권 제3호CD83 expression induced by CpG-DNA stimulation in a macrophage cell line RAW 264.7권형주, 김동범, 박민철, 이영희생화학분자생물학회 · BMB Reports2013 · 제46권 제9호

이 간행물의 다른 논문 (10건)

Mechanism of T cell exhaustion in a chronic environment정윤희, 박효진, Hyun-Tak Jin, 하상준pp. 217-231Current progress on development of respiratory syncytial virus vaccine장준pp. 232-237Functional analysis of expressed sequence tags from the liver and brain of Korean Jindo dogsJong Seok Kim, Seok Il Oh, Jae Young Kim, Hye Sun Park, Dajeong Lim, Hong Chul Jang, Hae Suk Park, Kyung Tai Lee, Mu Sik Kweon, Tae Hun Kim, Bong Hwan Choipp. 238-243Ex12 helper phage improves the quality of a phage-displayed antibody library by ameliorating the adverse effect of clonal variationsSang-hoon Cha, Suk-yoon Song, Li-kun Liu, Jae-bong Yoon, Hyo-jung Choi, Jae Youl Chopp. 244-249Predicting tissue-specific expressions based on sequence characteristicsSeung-Won Seo, 허철구, 이도헌, Hyojung Paik, Taewoo Ryu, Hyoung-Sam Heopp. 250-255
원문 보기