Activation of Toll-like receptor 9 and production of epitope specific antibody by liposome-encapsulated CpG-DNA
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Several investigators have shown that CpG-DNA has outstanding effects as a Th1-responsive adjuvant and that its potent adjuvant effects are enhanced by encapsulation with a liposome of proper composition. In this study, we showed that encapsulation with phosphatidyl-β-oleoyl-γ-palmitoyl ethanolamine (DOPE): cholesterol hemisuccinate (CHEMS) complex enhances the immunostimulatory activity of CpG DNA and the binding of CpG-DNA to TLR9. We also examined involvement of myeloid differentiation protein (MyD88) and NF-κB activation in liposome-encapsulated CpG-DNA-induced IL-8promoter activation. In this manuscript, the natural phosphodiester bond CpG-DNA encapsulated by DOPE : CHEMS complex is designated as Lipoplex(O). Importantly, we successfully screened B cell epitopes of envelope protein (E protein) of hepatitis C virus (HCV-E) and attachment glycoprotein G of human respiratory syncytial virus (HRSV-G) by immunization with complexes of several peptides and Lipoplex(O) without carriers. Therefore, Lipoplex(O) is potentially applicable as a universal adjuvant for peptide-based epitope screening and antibody production. [BMB reports 2011; 44(9): 607-612]
Abstract
Several investigators have shown that CpG-DNA has outstanding effects as a Th1-responsive adjuvant and that its potent adjuvant effects are enhanced by encapsulation with a liposome of proper composition. In this study, we showed that encapsulation with phosphatidyl-β-oleoyl-γ-palmitoyl ethanolamine (DOPE): cholesterol hemisuccinate (CHEMS) complex enhances the immunostimulatory activity of CpG DNA and the binding of CpG-DNA to TLR9. We also examined involvement of myeloid differentiation protein (MyD88) and NF-κB activation in liposome-encapsulated CpG-DNA-induced IL-8promoter activation. In this manuscript, the natural phosphodiester bond CpG-DNA encapsulated by DOPE : CHEMS complex is designated as Lipoplex(O). Importantly, we successfully screened B cell epitopes of envelope protein (E protein) of hepatitis C virus (HCV-E) and attachment glycoprotein G of human respiratory syncytial virus (HRSV-G) by immunization with complexes of several peptides and Lipoplex(O) without carriers. Therefore, Lipoplex(O) is potentially applicable as a universal adjuvant for peptide-based epitope screening and antibody production. [BMB reports 2011; 44(9): 607-612]
참고문헌 (20)
- Hemmi, H, "A Toll-like receptor recognizes bacterial DNA", Nature, 408, pp.740~745 (2000)
- Krieg, A. M, "CpG motifs in bacterial DNA trigger direct B cell activation", Nature, 374, pp.546~549 (1995)
- Ballas, Z. K, "Induction of NK activity in murine and human cells by CpG motifs in oligodeoxynucleotides and bacterial DNA", J. Immunol, 157, pp.1840~1845 (1996)
- Krieg, A. M, "CpG motifs in bacterial DNA and their immune effects", Annu. Rev. Immunol, 20, pp.709~760 (2002)
- Klinman, D. M, "CpG motifs present in bacteria DNA rapidly induce lymphocytes to secrete interleukin 6, interleukin 12, and interferon-γ", Proc. Natl. Acad. Sci. U.S.A, 93, pp.2879~2883 (1996)
- Dong, L, "An immunostimulatory oligodeoxynucleotide containing a cytidine-guanosine motif protects senescenceaccelerated mice from lethal influenza virus by augmenting the T helper type 1 response", J. Gen. Virol, 84, pp.1623~1628 (2003)
- 김두식, "Direct role of NF-κB activation in toll-like receptor-triggered HLA-DRA expression", EUROPEAN JOURNAL OF IMMUNOLOGY, 36, pp.1254~1266
- Ballas, Z. K, "Divergent therapeutic and immunologic effects of oligodeoxynucleotides with distinct CpG motifs", J. Immunol, 167, pp.4878~4886 (2001)
- Krieg, A. M, "Therapeutic potential of Toll-like receptor 9 activation", Nat. Rev. Drug. Discov, 5, pp.471~484 (2006)
- Heikenwalder, M, "Lymphoid follicle destruction and immunosuppression after repeated CpG oligodeoxynucleotide administration", Nat. Med, 10, pp.187~192 (2004)
- Deng, G. M, "Intra-articularly localized bacterial DNA containing CpG motifs induces arthritis", Nat. Med, 5, pp.702~705 (1999)
- Kim, D, "Immunostimulation and anti-DNA antibody production by backbone modified CpG-DNA", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 379(2), pp.362~367 (2009), ACADEMIC PRESS INC ELSEVIER SCIENCE
- 김두식, "Identification of Immunostimulatory Oligodeoxynucleotide from Escherichia coli Genomic DNA", BMB reports, 39(6), pp.788~793 (2006), 한국생화학분자생물학회
- Lee, K. W, "Immunostimulatory oligodeoxynucleotide isolated from genome wide screening of Mycobacterium bovis chromosomal DNA", MOLECULAR IMMUNOLOGY, 43(6), pp.2107~2118 (2006), 한국생화학분자생물학회
- Kim, D, "Production of antibodies with peptide-CpG-DNA-liposome complex without carriers", BMC IMMUNOLOGY, 12, pp.29-1~29-15 (2011), BIOMED CENTRAL LTD
- Zhao, Q, "Comparison of cellular binding and uptake of antisense phosphodiester, phosphorothioate, and mixed phosphorothioate and methylphosphonate oligonucleotides", Antisense Res. Dev, 36, pp.53~66 (1993)
- Zhao, Q, "Stage-specific oligonucleotide uptake in murine bone marrow B-cell precursors", Blood, 84, pp.3660~3666 (1994)
- Yasuda, K, "Endosomal translocation of vertebrate DNA activates dendritic cells via TLR9-dependent and -independent pathways", J. Immunol, 174, pp.6129~6136 (2005)
- Magnusson, M, "Natural DNA repetitive extragenic sequences from Gram-negative pathogens strongly stimulate TLR9", J. Immunol, 179, pp.31~35 (2007)
- Dongbum Kim, "Enhancement of immunomodulatory activity by liposome-encapsulated natural phosphodiester bond CpG-DNA in a human B cell line", BMB reports, 43(4), pp.250~256 (2010), 생화학분자생물학회