CD83 expression induced by CpG-DNA stimulation in a macrophage cell line RAW 264.7
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CpG-DNA has various immunomodulatory effects in dendritic cells, B cells, and macrophages. While induction of cytokines by CpG-DNA has been well documented in macrophages, the expression of costimulatory molecules in CpG-DNA treated macrophages has not yet been defined. Therefore, we investigated the effects of CpG-DNA on the expression of costimulatory molecules in RAW 264.7 cells. The surface expression of CD80 was slightly increased and CD83expression was significantly increased in response to CpG-DNA. However, the expression of CD86 and MHC class II was not changed. As expression of CD83 mRNA was also increased by CpG-DNA, CD83 expression is regulated at a transcriptional level. To understand the contribution of signaling pathways to CD83 induction, we used pathway specific inhibitors. The NF-κB inhibitor significantly reduced surface expression of CD83 as well as phagocytic activity of RAW 264.7 cells. Therefore, CD83 expression may contribute to the immunostimulatory effects of CpG-DNA in macrophage cells. [BMB Reports 2013; 46(9): 448-453]
Abstract
CpG-DNA has various immunomodulatory effects in dendritic cells, B cells, and macrophages. While induction of cytokines by CpG-DNA has been well documented in macrophages, the expression of costimulatory molecules in CpG-DNA treated macrophages has not yet been defined. Therefore, we investigated the effects of CpG-DNA on the expression of costimulatory molecules in RAW 264.7 cells. The surface expression of CD80 was slightly increased and CD83expression was significantly increased in response to CpG-DNA. However, the expression of CD86 and MHC class II was not changed. As expression of CD83 mRNA was also increased by CpG-DNA, CD83 expression is regulated at a transcriptional level. To understand the contribution of signaling pathways to CD83 induction, we used pathway specific inhibitors. The NF-κB inhibitor significantly reduced surface expression of CD83 as well as phagocytic activity of RAW 264.7 cells. Therefore, CD83 expression may contribute to the immunostimulatory effects of CpG-DNA in macrophage cells. [BMB Reports 2013; 46(9): 448-453]
참고문헌 (26)
- Janeway, Jr. C. A, "Introduction: the role of innate immunity in the adaptive immune response", Semin. Immunol, 10, pp.349~350 (1998)
- Aderem, A., "Toll-like receptors in the induction of the innate immune response", Nature, 406, pp.782~787 (2000)
- Anderson, K. V., "Toll signaling pathways in the innate immune response", Curr. Opin. Immunol, 12, pp.13~19 (2000)
- Akira, S., "Pathogen recognition and innate immunity", Cell, 124, pp.783~801 (2006)
- Hemmi, H., "A Toll-like receptor recognizes bacterial DNA", Nature, 408, pp.740~745 (2000)
- Krieg, A. M., "Therapeutic potential of Toll-like receptor 9 activation", Nat. Rev. Drug Discov, 5, pp.471~484 (2006)
- Stacey, K. J., "Macrophages ingest and are activated by bacterial DNA", J. Immunol, 157, pp.2116~2122 (1996)
- Yi, A. K., "CpG DNA rescue from anti-IgM-induced WEHI-231 B lymphoma apoptosis via modulation of IκBα and IκBβ and sustained activation of nuclear factor-κB", c-Rel, 1998, pp.160, 1240~1245
- Yi, A. K., "Rapid immune activation by CpG motifs in bacterial DNA. Systemic induction of IL-6 transcription through an antioxidant-sensitive pathway", J. Immunol, 157, pp.5394~5402 (1996)
- Jahrsdörfer, B., "CpG DNA increases primary malignant B cell expression of costimulatory molecules and target antigens", J. Leukoc. Biol, 69, pp.81~88 (2001)
- Hartmann, G., "CpG DNA: a potent signal for growth, activation, and maturation of human dendritic cells", Proc. Natl. Acad. Sci. U. S. A, 96, pp.9305~9312 (1999)
- Kadowaki, N., "Distinct CpG DNA and polyinosinic-polycytidylic acid double- stranded RNA, respectively, stimulate CD11c-type 2 dendritic cell precursors and CD11c+ dendritic cells to produce type I IFN", J. Immunol, 166, pp.2291~2295 (2001)
- Kadowaki, N., "Subsets of human dendritic cell precursors express different toll-like receptors and respond to different microbial antigens", J. Exp. Med, 194, pp.863~869 (2001)
- Krieg, A. M., "CpG motifs in bacterial DNA and their immune effects", Annu. Rev. Immunol, 20, pp.709~760 (2002)
- Dongbum Kim, "Adjuvant effect of liposome-encapsulated natural phosphodiester CpG-DNA", BMB Reports, 44(11), pp.758~763 (2011), 생화학분자생물학회
- Jiang, T., "CpG oligodeoxynucleotides protect against the 2009 H1N1 pandemic influenza virus infection in a murine model", Antiviral Res, 89, pp.124~126 (2009)
- Wong, J. P., "Activation of toll-like receptor signaling pathway for protection against influenza virus infection", Vaccine, 27, pp.3481~3483 (2009)
- Shoda, L. K., "Immunostimulatory CpG-modified plasmid DNA enhances IL-12, TNF-alpha, and NO production by bovine macrophages", J. Leukoc. Biol, 70, pp.103~112 (2001)
- Zhou, L. J., "A novel cell-surface molecule expressed by human interdigitating reticulum cells, Langerhans cells, and activated lymphocytes is a new member of the Ig superfamily", J. Immunol, 149, pp.735~742 (1992)
- Zhou, L. J., "Human blood dendritic cells selectively express CD83, a member of the immunoglobulin superfamily", J. Immunol, 154, pp.3821~3835 (1995)
- Caux, C., "Activation of human dendritic cells through CD40 cross-linking", J. Exp. Med, 180, pp.1263~1272 (1994)
- Zhou, L. J., "CD14+ blood monocytes can differentiate into functionally mature CD83+ dendritic cells", Proc. Natl. Acad. Sci. U. S. A, 93, pp.2588~2592 (1996)
- Fujimoto, Y., "CD83 expression influences CD4+ T cell development in the thymus", Cell, 108, pp.755~767 (2002)
- Breloer, M., "CD83 is a regulator of murine B cell function in vivo", Eur. J. Immunol, 37, pp.634~648 (2007)
- Lüthje, K., "CD83 regulates splenic B cell maturation and peripheral B cell homeostasis", Int. Immunol, 20, pp.949~960 (2008)
- Dongbum Kim, "Enhancement of immunomodulatory activity by liposome-encapsulated natural phosphodiester bond CpG-DNA in a human B cell line", BMB Reports, 43(4), pp.250~256 (2010), 생화학분자생물학회