Serial Changes of Heat Shock Protein 70 and Interleukin-8 in Burn Blister Fluid
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background: It has been reported that heat shock protein 70 (HSP70) and interleukin-8 (IL-8) play an important role in cells during the wound healing process. However, there has been no report on the effect of HSP70 and IL-8 on the blisters of burn patients. Objective: This study aimed to evaluate the serial quantitative changes of HSP70 and IL-8 in burn blisters. Methods: Twenty-five burn patients were included, for a total of 36 cases: twenty cases on the first day, six cases on the second, five cases on the third, three cases on the fourth, and two cases on the fifth. A correlation analysis was performed to determine the relationship between the concentration of HSP70 and IL-8 and the length of the treatment period. Results: The HSP70 concentration was the highest on the first day, after which it decreased down to near zero. Most HSP70 was generated during the first 12 hours after the burn accident. There was no correlation between the concentration of HSP70 on the first day and the length of the treatment period. No measurable concentration of IL-8 was detected before 5 hours, but the concentration started to increase after 11 hours. The peak value was measured on the fourth day. Conclusion: While HSP70 increased in the first few hours and decreased afterwards, IL-8 was produced after 11 hours and increased afterward in burn blister fluid. These findings provide new evidence on serial changes of inflammatory mediators in burn blister fluid.
Abstract
Background: It has been reported that heat shock protein 70 (HSP70) and interleukin-8 (IL-8) play an important role in cells during the wound healing process. However, there has been no report on the effect of HSP70 and IL-8 on the blisters of burn patients. Objective: This study aimed to evaluate the serial quantitative changes of HSP70 and IL-8 in burn blisters. Methods: Twenty-five burn patients were included, for a total of 36 cases: twenty cases on the first day, six cases on the second, five cases on the third, three cases on the fourth, and two cases on the fifth. A correlation analysis was performed to determine the relationship between the concentration of HSP70 and IL-8 and the length of the treatment period. Results: The HSP70 concentration was the highest on the first day, after which it decreased down to near zero. Most HSP70 was generated during the first 12 hours after the burn accident. There was no correlation between the concentration of HSP70 on the first day and the length of the treatment period. No measurable concentration of IL-8 was detected before 5 hours, but the concentration started to increase after 11 hours. The peak value was measured on the fourth day. Conclusion: While HSP70 increased in the first few hours and decreased afterwards, IL-8 was produced after 11 hours and increased afterward in burn blister fluid. These findings provide new evidence on serial changes of inflammatory mediators in burn blister fluid.
참고문헌 (30)
- Pan SC, "Deep partial thickness burn blister fluid promotes neovascularization in the early stage of burn wound healing", Wound Repair Regen, 18, pp.311~318 (2010)
- Ono I, "A study of cytokines in burn blister fluid related to wound healing", Burns, 21, pp.352~355 (1995)
- Widgerow AD, "Burn wound fluid: an important diagnostic source", Wound Healing South Afr, 7, pp.9~12 (2014)
- Pan SC, "Burn blister fluids in the neovascularization stage of burn wound healing: a comparison between superficial and deep partial-thickness burn wounds", Burns Trauma, 1, pp.27~31 (2013)
- Srivastava P, "Roles of heat-shock proteins in innate and adaptive immunity", Nat Rev Immunol, 2, pp.185~194 (2002)
- Giffard RG, "Regulation of apoptotic and inflammatory cell signaling in cerebral ischemia: the complex roles of heat shock protein 70", Anesthesiology, 109, pp.339~348 (2008)
- Henderson B, "Integrating the cell stress response: a new view of molecular chaperones as immunological and physiological homeostatic regulators", Cell Biochem Funct, 28, pp.1~14 (2010)
- Kennedy D, "Heat shock preconditioning protects against ER stress-induced apoptosis through the regulation of the BH3-only protein BIM", FEBS Open Bio, 4, pp.813~821 (2014)
- Sajjadi AY, "Expression of heat shock proteins 70 and 47 in tissues following short-pulse laser irradiation:assessment of thermal damage and healing", Med Eng Phys, 35, pp.1406~1414 (2013)
- Sokho Kim, "Thymosin β4 has a major role in dermal burn wound healing that involves actin cytoskeletal remodelling via heat-shock protein 70", Journal of Tissue Engineering and Regenerative Medicine, 11(4), pp.1262~1273 (2017), Wiley-Blackwell[DOI]
- Xiong L, "In vitro N-acetyl-L-cysteine promotes proliferation and suppresses interleukin-8 expression in adipose-derived stem cells", Aesthetic Plast Surg, 36, pp.1260~1265 (2012)
- Ashcroft GS, "Estrogen modulates cutaneous wound healing by downregulating macrophage migration inhibitory factor", J Clin Invest, 111, pp.1309~1318 (2003)
- Nwariaku F, "Inhibition of selectin- and integrin-mediated inflammatory response after burn injury", J Surg Res, 63, pp.355~358 (1996)
- Iocono JA, "Interleukin-8 levels and activity in delayed-healing human thermal wounds", Wound Repair Regen, 8, pp.216~225 (2000)
- Fujita T, "Interleukin-8 induces DNA synthesis, migration and down-regulation of cleaved caspase-3 in cultured human gingival epithelial cells", J Periodontal Res, 50, pp.479~485 (2015)
- Michel G, "Interleukin-8 receptor-mediated chemotaxis of normal human epidermal cells", FEBS Lett, 305, pp.241~243 (1992)
- Rennekampff HO, "Bioactive interleukin-8 is expressed in wounds and enhances wound healing", J Surg Res, 93, pp.41~54 (2000)
- Sargent RL, "Management of blisters in the partial-thickness burn: an integrative research review", J Burn Care Res, 27, pp.66~81 (2006)
- Cleland H, "Thermal burns--assessment and acute management in the general practice setting", Aust Fam Physician, 41, pp.372~375 (2012)
- duKamp A, "Deroofing minor burn blisters--what is the evidence?", Accid Emerg Nurs, 9, pp.217~221 (2001)
- Ireland HE, "Measuring the secretion of heat shock proteins from cells", Methods, 43, pp.176~183 (2007)
- Jong Youl Kim, "The immune modulating properties of the heat shock proteins after brain injury", Anatomy and Cell Biology, 46(1), pp.1~7 (2013), 대한해부학회
- Weissman C, "The metabolic response to stress: an overview and update", Anesthesiology, 73, pp.308~327 (1990)
- Ono SJ, "Chemokines: roles in leukocyte development, trafficking, and effector function", J Allergy Clin Immunol, 111, pp.1185~1199 (2003)
- Benkoe T, "Comprehensive evaluation of 11 cytokines in premature infants with surgical necrotizing enterocolitis", PLoS One, 8, pp.e58720~ (2013)
- Chan KY, "Immunoregulatory protein profiles of necrotizing enterocolitis versus spontaneous intestinal perforation in preterm infants", PLoS One, 7, pp.e36977~ (2012)
- Berney T, "Serum profiles of interleukin-6, interleukin-8, and interleukin-10 in patients with severe and mild acute pancreatitis", Pancreas, 18, pp.371~377 (1999)
- 이영복, "Immunomodulatory Effects of Deokgu Thermomineral Water Balneotherapy on Oxazolone-Induced Atopic Dermatitis Murine Model", Annals of Dermatology, 28(2), pp.192~198 (2016), 대한피부과학회
- Luo X, "Extracellular heat shock protein 70 inhibits tumour necrosis factor-alpha induced proinflammatory mediator production in fibroblast-like synoviocytes", Arthritis Res Ther, 10, pp.R41~ (2008)
- Krause M, "The chaperone balance hypothesis:the importance of the extracellular to intracellular HSP70ratio to inflammation-driven type 2 diabetes, the effect of exercise, and the implications for clinical management", Mediators Inflamm, 2015, pp.249205~ (2015)