Expression of a set of glial cell-specific markers in the Drosophila embryonic central nervous system
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The types of glia in the central nervous system (CNS) of theDrosophila embryo include longitudinal glia (LG), cell bodyglia (CBG), and peripheral glia (PG). Transcription factors, suchas glial cell missing and reverse polarity, are well-establishedgeneral glial cell markers. Only a few glial cell-specificmarkers have been identified in the Drosophila embryonicCNS, thus far. In the present study, we employed the glialcell-specific markers for LG (vir-1/CG5453 and CG31235),CBG (fabp/CG6783 and CG11902), and PG (CG2310 andmoody/CG4322), and comprehensively analyzed theirexpression patterns, during the embryonic CNS development. Our study validated the specificity of a set of glial markers, andfurther revealed their spatio-temporal expression patterns,which will aid in the understanding of the developmentallineage, and investigating their role in the development andhomeostasis of the Drosophila CNS in vivo.
Abstract
The types of glia in the central nervous system (CNS) of theDrosophila embryo include longitudinal glia (LG), cell bodyglia (CBG), and peripheral glia (PG). Transcription factors, suchas glial cell missing and reverse polarity, are well-establishedgeneral glial cell markers. Only a few glial cell-specificmarkers have been identified in the Drosophila embryonicCNS, thus far. In the present study, we employed the glialcell-specific markers for LG (vir-1/CG5453 and CG31235),CBG (fabp/CG6783 and CG11902), and PG (CG2310 andmoody/CG4322), and comprehensively analyzed theirexpression patterns, during the embryonic CNS development. Our study validated the specificity of a set of glial markers, andfurther revealed their spatio-temporal expression patterns,which will aid in the understanding of the developmentallineage, and investigating their role in the development andhomeostasis of the Drosophila CNS in vivo.
참고문헌 (21)
- Kettenmann, H., "Neuroglia" (2005), Oxford University Press
- Barres, B. A., "The mystery and magic of glia: A perspective on their roles in health and disease", Neuron, 60, pp.430~440 (2008)
- Allen, N. J., "Glia-more than just brain glue", Nature, 457, pp.675~677 (2009)
- 김민정, "Floridoside suppresses pro-inflammatory responses by blocking MAPK signaling in activated microglia", BMB Reports, 46(8), pp.398~403 (2013), 생화학분자생물학회
- 김지영, "Differential expression of αB-crystallin causes maturationdependent susceptibility of oligodendrocytes to oxidative stress", BMB Reports, 46(10), pp.501~506 (2013), 생화학분자생물학회
- Freeman, M. R., "Glial cell biology in Drosophila and vertebrates", Trends. Neurosci, 29, pp.82~90 (2006)
- Stork, T., "Cold Spring Harbor Protocols", pp.53~74 (2012), Cold Spring Harbor Laboratory Press
- Ito, K., "Distribution, classification, and development of Drosophila glial cells in the late embryonic and early larval ventral nerve cord", Roux's Arch. Dev. Biol, 204, pp.284~307 (1995)
- Beckervordersandforth, R. M., "Subtypes of glial cells in the Drosophila embryonic ventral nerve cord as related to lineage and gene expression", Mech. Dev, 125, pp.542~557 (2008)
- Hosoya, T., "glial cells missing: a binary switch between neuronal and glial determination in Drosophila", Cell, 82, pp.1025~1036 (1995)
- Jones, B. W., "glial cells missing: a genetic switch that controls glial versus neuronal fate", Cell, 82, pp.1013~1023 (1995)
- Vincent, S., "Glide directs glial fate commitment and cell fate switch between neurones and glia", Development, 122, pp.131~139 (1996)
- Egger, B., "Gliogenesis in Drosophila: genome-wide analysis of downstream genes of glial cells missing in the embryonic nervous system", Development, 129, pp.3295~3309 (2002)
- Freeman, M. R., "Unwrapping glial biology: Gcm target genes regulating glial development, diversification, and function", Neuron, 38, pp.567~580 (2003)
- Altenhein, B., "Expression profiling of glial genes during Drosophila embryogenesis", Dev. Biol, 296, pp.545~560 (2006)
- Saleh, M. C., "Antiviral immunity in Drosophila requires systemic RNA interference spread", Nature, 458, pp.346~350 (2009)
- Stacey, S. M., "Drosophila glial glutamate transporter eaat1 is regulated by fringe-mediated notch signaling and is essential for larval locomotion", J. Neurosci, 30, pp.14446~14457 (2010)
- Bainton, R. J., "moody encodes two GPCRs that regulate cocaine behaviors and blood-brain barrier permeability in Drosophila", Cell, 123, pp.145~156 (2005)
- Schwabe, T., "GPCR signaling is required for blood-brain barrier formation in Drosophila", Cell, 123, pp.133~144 (2005)
- O’Neill, J. W, "Double in situ hybridization using biotin and digoxigenin tagged RNA probes", Biotechniques, 17, pp.873~875 (1994)
- Lehmann, R., "In situ hybridization to RNA; in Methods in Cell Biology Vol. 44", pp.576~597 (1994), Academic Press