Replication and packaging of Turnip yellow mosaic virus RNA containing Flock house virus RNA1 sequence
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Turnip yellow mosaic virus (TYMV) is a spherical plant virus that has a single 6.3 kb positive strand RNA as a genome. Inthis study, RNA1 sequence of Flock house virus (FHV) wasinserted into the TYMV genome to test whether TYMV canaccommodate and express another viral entity. In the resultingconstruct, designated TY-FHV, the FHV RNA1 sequence wasexpressed as a TYMV subgenomic RNA. Northern analysis ofthe Nicotiana benthamiana leaves agroinfiltrated with theTY-FHV showed that both genomic and subgenomic FHVRNAs were abundantly produced. This indicates that the FHVRNA1 sequence was correctly expressed and translated toproduce a functional FHV replicase. Although these FHVRNAs were not encapsidated, the FHV RNA having a TYMVCP sequence at the 3’-end was efficiently encapsidated. Whenan eGFP gene was inserted into the B2 ORF of the FHVsequence, a fusion protein of B2-eGFP was produced as expected.
Abstract
Turnip yellow mosaic virus (TYMV) is a spherical plant virus that has a single 6.3 kb positive strand RNA as a genome. Inthis study, RNA1 sequence of Flock house virus (FHV) wasinserted into the TYMV genome to test whether TYMV canaccommodate and express another viral entity. In the resultingconstruct, designated TY-FHV, the FHV RNA1 sequence wasexpressed as a TYMV subgenomic RNA. Northern analysis ofthe Nicotiana benthamiana leaves agroinfiltrated with theTY-FHV showed that both genomic and subgenomic FHVRNAs were abundantly produced. This indicates that the FHVRNA1 sequence was correctly expressed and translated toproduce a functional FHV replicase. Although these FHVRNAs were not encapsidated, the FHV RNA having a TYMVCP sequence at the 3’-end was efficiently encapsidated. Whenan eGFP gene was inserted into the B2 ORF of the FHVsequence, a fusion protein of B2-eGFP was produced as expected.
참고문헌 (19)
- Dreher, T. W., "Turnip yellow mosaic virus: transfer RNA mimicry, chloroplasts and a C-rich genome", Mol. Plant Pathol, 5, pp.367~375 (2004)
- Prod’homme, D., "Targeting of the Turnip Yellow Mosaic Virus 66K replication protein to the chloroplast envelope is mediated by the 140K protein", J. Virol, 77, pp.9124~9135 (2003)
- Chen, J., "Viral virulence protein suppresses RNA silencing- mediated defense but upregulates the role of microRNA in host gene expression", Plant Cell, 16, pp.1302~1313 (2004)
- Yildiz, I., "Applications of viral nanoparticles in medicine", Curr. Opin. Biotechnol, 22, pp.901~908 (2011)
- Hyun-Il Shin, "Replication and encapsidation of recombinant Turnip yellow mosaic virus RNA", BMB reports, 41(10), pp.739~744 (2008), 한국생화학분자생물학회
- Ball, L. A., "Requirements for the self-directed replication of Flock house virus RNA1", J. Virol, 69, pp.720~727 (1995)
- Li, H., "Induction and suppression of RNA silencing by an animal virus", Science, 296, pp.1319~1321 (2002)
- Selling, B. H., "Genomic RNA of an insect virus directs synthesis of infectious virions in plants", Proc. Natl. Acad. Sci, 87, pp.434~438 (1990)
- Price, B. D., "Complete replication of an animal virus and maintenance of expression vectors derived from it in Saccharomyces cerevisiae", Proc. Natl. Acad. Sci, 93, pp.9465~9470 (1996)
- Johnson, K. L., "Induction and maintenance of autonomous Flock house virus RNA1 replication", J. Virol, 73, pp.7933~7942 (1999)
- Perrotta, A. T., "Imidazole rescue of a cytosine mutation in a self-cleaving ribozyme", Science, 286, pp.123~126 (1999)
- Schirawski, J., "Identification and functional analysis of the turnip yellow mosaic tymovirus subgenomic promoter", J. Virol, 74, pp.11073~11080 (2000)
- Annamalai, P., "Replication-coupled packaging mechanism in positive- strand RNA viruses: Synchronized coexpression of functional multigenome RNA components of an animal and a plant virus in Nicotiana benthamiana cells by agroinfiltraion", J. Virol, 82, pp.1484~1495 (2008)
- Shin, H. -I., "The Pro", Hel Region is indispensable for packaging non-replicating Turnip Yellow Mosaic Virus RNA, but not replicating viral RNA. Mol, 2010, pp.29, 463~469
- Miller, D. J., "Flock house virus RNA replicates on outer mitochondrial membranes in Drosophila cells", J. Virol, 75, pp.11664~11676 (2001)
- Price, B. D., "DNA-directed expression of functional flock house virus RNA1 derivatives in Saccharomyces cerevisiae, heterologous gene expression, and selective effects on subgenomic mRNA synthesis", J. Virol, 74, pp.11724~11733 (2000)
- Cho, T.-J., "Encapsidation of genomic but not subgenomic Turnip yellow mosaic virus RNA by coat protein provided in trans", Virology, 356, pp.126~135 (2006)
- 신현일, "Modification of Turnip yellow mosaic virus coat protein and its effect on virion assembly", BMB Reports, 46(10), pp.495~500 (2013), 생화학분자생물학회
- 신순영, "Egr-1 regulates the transcription of the BRCA1 gene by etoposide", BMB Reports, 46(2), pp.92~96 (2013), 생화학분자생물학회