Cloning and Spatiotemporal Expression Analysis of Bombyx mori elav, an Embryonic Lethal Abnormal Visual Gene
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Embryonic lethal abnormal visual (elav) is a lethal gene in Drosophila inducing the abnormal development and function of nervous system. We cloned a Bmelav gene by bioinformatics and biological experiment, based on sequence of ELAV protein and dbEST of Bombyx mori. The full-length of Bm-elav cDNA is 1498 bp, contains a 906 bp open read frame (ORF) encoding a precursor of 301 amino acid residues with a calculated molecular weight of 34 kDa and pI of 8.99. Bm-ELAV protein precursor contains three RNA recognition motifs (RRM) in 24~91, 110~177 and 222~295 bit amino acid residues respectively, and belongs to RNA-binding protein family. Bm-ELAV shared varying positives, ranging from 56% to 60% (Identities from 41% to 45%), with RRM from other species of Xenopus tropicalis, Apis mellifera, Tribolium castaneum, Branchiostoma belcheri and Drosophila. Gene localization indicated that Bm-elav is a single-copy gene, gene mapping within 12-chromosome from 7916.68 knt to 7918.16 knt region of nscaf2993. Spatiotemporal expressions pattern analysis revealed that Bm-elav expressed higher in most tested tissues and developmental stages in whole generation, such as silk gland, fat body, midgut, hemopoietic organ and ovary, but almost no expression in terminated diapause eggs. This suggested that the expression of Bm-elav in early developmental embryonic stages might induce abnormal development like in Drosophila. Cloning of the Bmelav gene enables us to test its potential role in controlling pests by transferring the gene into field lepidopteran insects in the future.
Abstract
Embryonic lethal abnormal visual (elav) is a lethal gene in Drosophila inducing the abnormal development and function of nervous system. We cloned a Bmelav gene by bioinformatics and biological experiment, based on sequence of ELAV protein and dbEST of Bombyx mori. The full-length of Bm-elav cDNA is 1498 bp, contains a 906 bp open read frame (ORF) encoding a precursor of 301 amino acid residues with a calculated molecular weight of 34 kDa and pI of 8.99. Bm-ELAV protein precursor contains three RNA recognition motifs (RRM) in 24~91, 110~177 and 222~295 bit amino acid residues respectively, and belongs to RNA-binding protein family. Bm-ELAV shared varying positives, ranging from 56% to 60% (Identities from 41% to 45%), with RRM from other species of Xenopus tropicalis, Apis mellifera, Tribolium castaneum, Branchiostoma belcheri and Drosophila. Gene localization indicated that Bm-elav is a single-copy gene, gene mapping within 12-chromosome from 7916.68 knt to 7918.16 knt region of nscaf2993. Spatiotemporal expressions pattern analysis revealed that Bm-elav expressed higher in most tested tissues and developmental stages in whole generation, such as silk gland, fat body, midgut, hemopoietic organ and ovary, but almost no expression in terminated diapause eggs. This suggested that the expression of Bm-elav in early developmental embryonic stages might induce abnormal development like in Drosophila. Cloning of the Bmelav gene enables us to test its potential role in controlling pests by transferring the gene into field lepidopteran insects in the future.
참고문헌 (17)
- Adams MD., "The genome sequence of Drosophila melanogaster", Science, 287, pp.2185~2195 (2000)
- Antic D., "Insights from model systems: Embryonic lethal abnormal visual RNA-binding proteins involved in growth, differentiation, and posttranscriptional gene expression", Am J Hum Genet, 61, pp.273~278 (1997)
- Borgeson CD., "Shared RNA-binding sites for interacting members of the Drosophila ELAV family of neuronal proteins", Nucleic Acids Res., 33, pp.6372~6383 (2005)
- Burd CG., "Conserved structures and diversity of functions of RNA-binding proteins", Science, 265, pp.615~621 (1994)
- Campos AR., "Molecular analysis of the locus elav in Drosophila melanogaster: a gene whose embryonic expression is neural specific", EMBO J., 6, pp.425~431 (1987)
- Gill RW., "Rapid in silico cloning of genes using expressed sequence tags (ESTs)", Biotechnol Annu Rev., 5, pp.25~44 (2000)
- Goldsmith MR., "Molecular Model Systems in the Lepidoptera", pp.21~76 (1995), Cambridge University Press
- Gong P., "A dominant lethal genetic system for autocidal control of the Mediterranean fruit fly", Nature Biotechnol, 23, pp.453~456 (2005)
- Good PJ., "Conserved family of elav-like genes in vertebrates", Proc Natl Acad Sci USA, 92, pp.4557~4561 (1995)
- Manley GT., "Hu antigens: reactivity with Hu antibodies, tumor expression, and major immunogenic sites", Ann Neurol., 38, pp.102~110 (1995)
- Miao XX., "Simple se-quence repeat-based consensus linkage map of Bombyx mori", Proc Natl Acad Sci USA, 102, pp.16303~16308 (2005)
- Mita K., "The genome sequence of silkworm, Bombyx mori", DNA Research, 11, pp.27~35 (2004)
- Robinow S., "The elav gene product of Drosophila, required in neurons, has three RNP consensus motifs", Science, 242, pp.1570~1572 (1988)
- Robinow S., "Characterization and spatial distribution of the ELAV protein during Drosophila melanogaster development", J Neurobiol., 22, pp.443~461 (1991)
- Simionato E., "The Drosophila RNA-binding protein ELAV is required for commissural axon midline crossing via control of commissureless mRNA expression in neurons", Dev Biol., 301, pp.166~177 (2007)
- Thomas DT., "Insect population control using a dominant, repressible, lethal genetic system", Science, 287, pp.2474~2476 (2000)
- Xia Q., "A draft sequence for the genome of the domesticated silkworm (Bombyx mori)", Science, 306, pp.1937~1940 (2004)