Genome-wide in-locus epitope tagging of Arabidopsis proteins using prime editors
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Prime editors (PEs), which are CRISPR-Cas9 nickase (H840A)-reverse transcriptase fusion proteins programmed with primeediting guide RNAs (pegRNAs), can not only edit bases butalso install transversions, insertions, or deletions without bothdonor DNA and double-strand breaks at the target DNA. Asthe demand for in-locus tagging is increasing, to reflect geneexpression dynamics influenced by endogenous genomic contexts,we demonstrated that PEs can be used to introduce thehemagglutinin (HA) epitope tag to a target gene locus, enablingmolecular and biochemical studies using in-locus taggedplants. To promote genome-wide in-locus tagging, we also implementeda publicly available database that designs pegRNAsfor in-locus tagging of all the Arabidopsis genes.
Abstract
Prime editors (PEs), which are CRISPR-Cas9 nickase (H840A)-reverse transcriptase fusion proteins programmed with primeediting guide RNAs (pegRNAs), can not only edit bases butalso install transversions, insertions, or deletions without bothdonor DNA and double-strand breaks at the target DNA. Asthe demand for in-locus tagging is increasing, to reflect geneexpression dynamics influenced by endogenous genomic contexts,we demonstrated that PEs can be used to introduce thehemagglutinin (HA) epitope tag to a target gene locus, enablingmolecular and biochemical studies using in-locus taggedplants. To promote genome-wide in-locus tagging, we also implementeda publicly available database that designs pegRNAsfor in-locus tagging of all the Arabidopsis genes.
참고문헌 (24)
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