Identification of optimal adenine and cytosine base editors for genome editing in Arabidopsis and soybean
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Base editors, including adenine base editors (ABEs) and cytosinebase editors (CBEs), are widely used in numerous organisms tointroduce site-specific sequence modifications in genomic DNAwithout causing double-strand breaks (DSBs). However, theseeditors exhibit low editing efficiencies, particularly in dicotplants, thereby limiting their application in dicot plant genomeengineering. In this study, we assessed the editing efficienciesof various base editors to identify those optimal for base editingin dicot plants. We discovered that ABE8e, an ABE variant,demonstrated superior A-to-G base editing efficiency withinA5-A8 windows, and A3A/Y130F-V04, a CBE variant, exhibitedthe highest C-to-T base editing efficiency within C4-C15windows in both Arabidopsis and soybean protoplasts. Overall,we recommend these two base editors as prime choices forefficient genome engineering in a range of crop plants.
Abstract
Base editors, including adenine base editors (ABEs) and cytosinebase editors (CBEs), are widely used in numerous organisms tointroduce site-specific sequence modifications in genomic DNAwithout causing double-strand breaks (DSBs). However, theseeditors exhibit low editing efficiencies, particularly in dicotplants, thereby limiting their application in dicot plant genomeengineering. In this study, we assessed the editing efficienciesof various base editors to identify those optimal for base editingin dicot plants. We discovered that ABE8e, an ABE variant,demonstrated superior A-to-G base editing efficiency withinA5-A8 windows, and A3A/Y130F-V04, a CBE variant, exhibitedthe highest C-to-T base editing efficiency within C4-C15windows in both Arabidopsis and soybean protoplasts. Overall,we recommend these two base editors as prime choices forefficient genome engineering in a range of crop plants.