Rapid Detection of Pathogens Associated with Dental Caries and Periodontitis by PCR Using a Modified DNA Extraction Method
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초록
DNA extraction is a prerequisite for the identification of pathogens in clinical samples. Commercial DNA extractionkits generally involve time-consuming and laborious multi-step procedures. In the present study, our modified DNA isolation method for saliva samples allows for the quick detection ofpathogens associated with dental caries or periodontitis by PCR within 1 h. To release DNA from the bacteria, 1min of boiling was adequate, and the resulting isolated DNA can be used many times and is suitable for longterm storage of at least 13 months at 4℃, and even longer at -20℃. In conclusion, our modified DNA extraction method is simple, rapid, and cost-effective, and suitable forpreparing DNA from clinical samples for PCR for the rapid detection of oral pathogens from saliva.
Abstract
DNA extraction is a prerequisite for the identification of pathogens in clinical samples. Commercial DNA extractionkits generally involve time-consuming and laborious multi-step procedures. In the present study, our modified DNA isolation method for saliva samples allows for the quick detection ofpathogens associated with dental caries or periodontitis by PCR within 1 h. To release DNA from the bacteria, 1min of boiling was adequate, and the resulting isolated DNA can be used many times and is suitable for longterm storage of at least 13 months at 4℃, and even longer at -20℃. In conclusion, our modified DNA extraction method is simple, rapid, and cost-effective, and suitable forpreparing DNA from clinical samples for PCR for the rapid detection of oral pathogens from saliva.
참고문헌 (14)
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