Comparison of quantitative detection of periodontal pathogens before and after scaling by real-time polymerase chain reaction
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Objectives: The purpose of the study is to investigate the quantitative detection of periodontal pathogens before and after scaling by real-time polymerase chain reaction. Methods: Participants were voluntarily recruited at D university, and saliva samples were extracted before and after scaling. Multiple real-time polymerase chain reactions were used to analyze characteristics and the amount of nine kinds of periodontal pathogens; Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Prevotella intermedia, Fusobacterium nucleatum, Parvimonas micra, Campylobacter rectus, and Eikenella corrodens. Results: After scaling, most periodontal pathogens except Eikenella corrodens were significantly decreased in all subjects(p<0.05). In addition, the percentage of microorganisms associated with disease, the microorganism risk index of periodontitis and the prevalence of red complex, orange complex, and Aggregatibacter actinomycetemcomitans was also significantly reduced after scaling(p<0.05). Conclusions: Scaling decreased in the amount of major periodontal pathogens and periodontitis prevalence rate.
Abstract
Objectives: The purpose of the study is to investigate the quantitative detection of periodontal pathogens before and after scaling by real-time polymerase chain reaction. Methods: Participants were voluntarily recruited at D university, and saliva samples were extracted before and after scaling. Multiple real-time polymerase chain reactions were used to analyze characteristics and the amount of nine kinds of periodontal pathogens; Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Prevotella intermedia, Fusobacterium nucleatum, Parvimonas micra, Campylobacter rectus, and Eikenella corrodens. Results: After scaling, most periodontal pathogens except Eikenella corrodens were significantly decreased in all subjects(p<0.05). In addition, the percentage of microorganisms associated with disease, the microorganism risk index of periodontitis and the prevalence of red complex, orange complex, and Aggregatibacter actinomycetemcomitans was also significantly reduced after scaling(p<0.05). Conclusions: Scaling decreased in the amount of major periodontal pathogens and periodontitis prevalence rate.
참고문헌 (29)
- 정유진, "성인의 치아우식증 및 치주질환에 영향을 미치는 요인", 한국치위생학회지, 15(1), pp.47~54 (2015), 한국치위생학회[DOI]
- Mohangi GU, "Periodontal disease: mechanisms of infection and inflammation and possible impact on miscellaneous systemic diseases and conditions", SADJ, 68, pp.462~467 (2013)
- van Houte J, "Role of micro-organisms in caries etiology", J Dent Res, 73, pp.672~681 (1994)
- Petersen PE, "WHO's action for continuous improvement in oral health", Bull World Health Organ, 83, pp.642~ (2005)
- Lopez R, "On putative periodontal pathogens: an epidemiological perspective", Virulence, 6, pp.249~257 (2015)[DOI]
- Theilade E, "Advances in oral microbiology", Ann R Australas Coll Dent Surg, 10, pp.62~71 (1989)
- Marchesan J, "TLR4, NOD1 and NOD2 mediate immune recognition of putative newly identified periodontal pathogens", Mol Oral Microbiol (2015)[DOI]
- Salminen A, "Quantitative PCR analysis of salivary pathogen burden in periodontitis", Front Cell Infect Microbiol, 5, pp.69~ (2015)[DOI]
- Chahboun H, "Bacterial profile of aggressive periodontitis in Morocco: a cross-sectional study", BMC Oral Health, 15, pp.25~ (2015)[DOI]
- Socransky SS, "Microbial complexes in subgingival plaque", J Clin Periodontol, 25, pp.134~144 (1998)
- Ximénez-Fyvie LA, "Microbial composition of supra- and subgingival plaque in subjects with adult periodontitis", J Clin Periodontol, 27, pp.722~732 (2000)
- Haubek D, "The highly leukotoxic JP2 clone of Actinobacillus actinomycetemcomitans and progression of periodontal attachment loss", J Dent Res, 83, pp.767~770 (2004)
- Arabaci T, "Sonic and ultrasonic scalers in periodontal treatment: a review", Int J Dent Hyg, 5, pp.2~12 (2007)
- Petersilka GJ, "Antimicrobial effects of mechanical debridement", Periodontol 2000, 28, pp.56~71 (2002)
- Darby I, "Non-surgical management of periodontal disease", Aust Dent J, 54, pp.S86~S95 (2009)[DOI]
- Cobb CM, "Clinical significance of non-surgical periodontal therapy: an evidence-based perspective of scaling and root planing", J Clin Periodontol, 29, pp.6~16 (2002)
- Faveri M, "Scaling and root planing and chlorhexidine mouthrinses in the treatment of chronic periodontitis: a randomized, placebo-controlled clinical trial", J Clin Periodontol, 33, pp.819~828 (2006)
- Heitz-Mayfield LJ, "A systematic review of the effect of surgical debridement vs non-surgical debridement for the treatment of chronic periodontitis", J Clin Periodontol, 29, pp.92~102 (2002)
- Haffajee AD, "Clinical and microbiological features of subjects with adult periodontitis who responded poorly to scaling and root planing", J Clin Periodontol, 24, pp.767~776 (1997)
- Knöfler GU, "Microbiologic findings 1 year after partialand full-mouth scaling in the treatment of moderate chronic periodontitis", Quintessence Int, 42, pp.e107~e117 (2011)
- Yang H, "Association between Porphyromonas gingivalis and scaling and root planning therapy", Hua Xi Kou Qiang Yi Xue Za Zhi, 26, pp.147~151 (2008)
- Braga RR, "Quantification of five putative periodontal pathogens in female patients with and without chronic periodontitis by real-time polymerase chain reaction", Anaerobe, 16, pp.234~239 (2010)[DOI]
- Salminen A, "Quantitative PCR analysis of salivary pathogen burden in periodontitis", Front Cell Infect Microbiol, 5, pp.69~ (2015)[DOI]
- 김선미, "Real-time PCR을 이용한 치주질환 원인균의 정량적 분석", 대한소아치과학회지, 35(3), pp.494~503 (2008), 대한소아치과학회
- Lee MR, "A study on the change of oral microorganism before and after oral hygiene management", Korea Academy of Mental Health Social Work, 13, pp.51~65 (2011)
- 조홍범, "Multiplex Real-Time PCR을 이용하여 6종의 주요 잇몸질환 유발 미생물을 동시에 검출하는 기법", 미생물학회지, 49(3), pp.292~296 (2013), 한국미생물학회[DOI]
- Geneall Biotechnology, "DNA purification handbook", pp.11~38 (2012), Geneall biotechnology
- Könönen E, "Population-based study of salivary carriage of periodontal pathogens in adults", J Clin Microbiol, 45, pp.2446~2451 (2007)[DOI]
- 김미광, "Comparison of Detection Rate of Prevotella intermedia and Prevotella nigrescens in Subgingival Plaque from Korean Periodontitis Patients", 대한구강보건학회지, 28(2), pp.279~284 (2004), 대한예방치과·구강보건학회