셀레늄이 전리방사선에 의한 힌쥐 모델에서의 갑상선 항산화계에 미치는 영향
Effect of Selenium on the Thyroid gland Antioxidative Metabolisms in Rat Model by Ionizing Radiation
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
천연물에 존재하는 셀레늄(Se)은 생체의 산화환원작용을 주관하는 중요한 단백질의 하나인 셀레늄함유단백질(selenoprotein)의 중요한 요소로 알려져 있다. 셀레늄(Se)을 Rat에 경구 투여하여 10 Gy의 방사선을 조사 시킨 후갑상선을 표적 장기로 삼고 1일, 7일, 21일 기간에 따른 혈구성분의 변화, 갑상선호르몬(T3, T4)의 변화, 항산화효소((Glutathione Peroxidase, GPx)의 활성 변화, 갑상선 조직 변화 관찰을 통하여 셀레늄(Se)의 방사선 방호 작용을 알아보고자 하였다. 실험결과 조혈 면역계(혈색소농도, 호중구, 혈소판)에서 회복을 보이는 유의한 방호 효과가 있었다(p<0.05). 항산화효소인 Glutathione Peroxidase(GPx) 활성과 표적 장기인 갑상선 호르몬(T3, T4)의 활성 변화 결과에서도 유의성 있는 활성 변화를 보였으며(p<0.05), 조직 변화 관찰에서는 방사선 처리에 의한 세포 괴사를 일으킨 갑상선세포 손상 보호 효과가 있음을 확인하였다. 따라서, 셀레늄(Se)은 떨어진 생체의 면역 활성 효과를 유도함으로써방사선 방어제로 활용될 수 있을 것이라 판단된다.
Abstract
Selenium (Se), which is natural materials existing was known as an important component of selenoprotein, one of the important proteins responsible for the redox pump of a living body. Selenium was orally administered to Rat and irradiated with 10 Gy of radiation. Then, the thyroid gland was used as a target organ for 1 day, 7 days and 21 days to investigate the radiation protection effect of selenium (Se) through changes of blood components, thyroid hormones (T3, T4), antioxidant enzyme (GPx) activity and thyroid tissue changes. As a result, there was a significant protective effect of hematopoietic immune system(hemoglobin concentration, neutrophil, platelet)(p<0.05). The activity of Glutathione Peroxidase (GPx), the antioxidant enzyme, and the activity of the target organ, thyroid hormone (T3, T4), also showed significant activity changes (p<0.05). In the observation of tissue changes, it was confirmed that there was a protective effect of thyroid cell damage which caused the cell necrosis by radiation treatment. Therefore, it is considered that selenium(Se) can be utilized as a radiation defense agent by inducing immunogenic activity effect of a living body.
참고문헌 (26)
- 김종순, "갑상선암과 방사선", International Journal of Thyroidology, 8(1), pp.1~7 (2015), 대한갑상선학회
- Rayman M. P., "The importance of selenium to human health", The Lancet, 356, pp.233~241 (2000)
- J. H. Park, "Cancer Facts & Figures", pp.6~15 (2012), National Cancer Center, Ministry of Health & Welfare
- Sunde RA, "Molecular biology of selenoproteins", Annu Rev Nutr, 10, pp.451~474 (1990)
- Duntas LH, "Effects of a six month treatment with selenomethionine in patients with autoimmune thyroiditis", Eur J Endocrinol, 148, pp.389~393 (2003)
- Turker O, "Selenium treatment in autoimmune thyroiditis:9-month followup with variable doses", J Endocrinol, 190, pp.151~156 (2006)
- Paglia DE, "Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase", J Lab Clin Med, 70, pp.158~169 (1967)
- Sources and Effects of Ionizing Radiation(UNSCRAR 2008 Report to the General Assembly with Scientific Annexes C, D and E) Vol. II, "United Nations Scientific Committee on the Effects of Atomic Radiation", United Nations, ~ (2011)
- Ivanov VK, "Thyroid cancer incidence among adolescents and adults in the Bryansk region of Russia following the Chernobyl accident", Health Phys, 84(1), pp.46~60 (2003)
- "Health effects of the Chernobyl accident and special health care programmes", Chernobyl Forum (2006), Geneva World Health Organization
- Dickman PW, "Thyroid cancer risk after thyroid examination with 131I apopulation-based cohort study in Sweden", Int J Cancer, 106(4), pp.580~587 (2003)
- Ivanov VK, "Radiation-epidemiological studies of thyroid cancer incidence in Russia after the Chernobyl accident(estimation of radiation risks, 1991-2008 follow-up period)", Radiat Prot Dosimetry, 151(3), pp.489~499 (2012)
- Birringer M, "Trends in selenium biochemistry", Nat Prod Rep, 19, pp.693~718 (2002)
- Behne D, "Mammalian selenium containing proteins", Annu Rev Nutr, 21, pp.453~473 (2001)
- Dong-Hwan LEE, "Inherited Metabolic Diseases", pp.775~776 (2008), KOMB
- Larsen PR, "Update on the human iodothyronine selenodeiodinases, the enzymes regulating the activation and inactivation of thyroid hormone", Biochem Soc Trans, 25, pp.588~592 (1997)
- Windisch W, "Effect of selenite, selonocystein and selenomethionine on the selenium metabolism of 75Se labeled rat", Anim. Physiol. a. Anim Nutr, 78, pp.67~74 (1997)
- Lawrence, R. A, "Species, tissue and subcellular distribution of non Se-dependent glutathione peroxidase", J. Nutr, 108, pp.211~215 (1978)
- Rayman M. P., "The importance of selenium to human health", The Lancet, 356, pp.233~241 (2000)
- Navarro- Alarcon M, "Essentiality of selenium in the human body: relationship with different diseases", Sci. of Tot. Environ, 249, pp.347~371 (2000)
- 김현하, "우리나라 일부 영아의 혈액 셀레늄과 Glutathione Peroxidase 효소 활성에 관한 연구", Journal of Nutrition and Health, 44(2), pp.112~118 (2011), 한국영양학회
- Marklund S., "Involvement of superoxide anon radical in the oxidaton of pyrogalloland a convenient assay for superoxide dismutase", Eur J Biochem, 47, pp.468~ (1974)
- Zimnitsky B., "The effect of the X-ray upon the fine structure of the parenchyma of the thyroid gland(1st article)", Radiology, 27, pp.68~ (1936)
- Eckert C. T., "Radiation of the thyroid: An experimental study in radiosensitivity of the thyroid", Radiology, 29, pp.40~ (1937)
- Warrens S., "Effects of radiation on normal tissues", Arch. path, 35, pp.304~ (1943)
- Zimnitsky B., "The effect of the X-ray upon the fine structure of the parenchyma of the thyroid gland(1st article)", Radiology, 27, pp.175~ (1936)