전신 X-선 조사 후 마우스의 대동맥 혈구에서 SEM을 이용한 프로폴리스의 방사선방어효과 연구
Radioprotective Effect of Propolis on the Blood Corpuscle of a Mouse by SEM after X-irradiation on the Whole Body
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초록
선형가속기를 이용한 X-선 5Gy를 마우스 모델에 전신 조사 후 백혈구, 적혈구, 혈소판을 주사전자현미경으로 관찰하였다. 또한 프로폴리스를 복강 주사 후 혈구들의 방사선 방어효과를 알아보았다. 혈구의 미세구조 관찰에서는 외관상 방사선 조사한 실험군에서 수가 줄어들었으며, 조사 후 10일에서 20일로 경과함에 따라 혈구의 엉김이 관찰되었다. 적혈구에서는 표면이 균열이 생기고 깨어진 형태와 변성구상적혈구들이 관찰되었다. 백혈구는 방사선조사군에서 혈구의 크기가 줄어들었으며, 가장자리 미융모들의 크기와 수가 대조군 보다 작은 것으로 관찰되었다. 프로폴리스 투여 후 방사선 조사군에서는 혈구들이 대조군과 유사한 형태로 확인되었다.
Abstract
After x-ray 5Gy radiation on the whole body of a mouse using a linear accelerator, its leucocyte, erythrocyte, and platelet were observed by SEM. Also, after injecting propolis into the abdominal cavity, the radio-protective effect of blood corpuscles was studied. The observation of micromorphology in blood corpuscles revealed that the number of leukocyte, erythrocyte, and thrombocyte decreased in the experimental group and the lump got together in blood corpuscles after 10 and 20 days. In RBC, crack or break on the surface and poikilosperocytes were observed. In the irradiation group, the size of leucocytes was smaller than that in control group and the number of villus at the verge substantially decreased. The blood corpuscles in the propolis group, however, had the similar results to control group.
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