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논문KCI 등재

Mouse에 있어서 시험물질 淸心蓮子湯 추출액의 급성독성 시험

Acute Toxicity Study on Cheongsimyeonja-tang in Mice

서창섭|마진열
사상체질면역의학회 (The Society of Sasang Constitution and Immune Medicine)|2010.03|사상체질면역의학회지 (Journal of Sasang Constitution and Immune Medicine)|제22권 제1호|pp. 59-65

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참고문헌

키워드

LD50Acute ToxicityCheongsimyeonja-tang(Qingxinlianzi-tang)Toxicity Test
주제분류한의학

초록

1. Objectives The aim of this study is data analysis for acute toxicity and safety of Cheongsimyeonja-tang. 2. Methods We investigated the acute toxicity for water-extracted Cheongsimyeonja-tang. Fifty five male and female mice were observed for 14 days after one day oral administration of Cheongsimyeonja-tang at the respective doses of 0 (control group), 2560, 3200, 4000 and 5000 ㎎/㎏. 3. Results We observed survival rates, general toxicity, change of body weight and autopsy. In animals administered with Cheongsimyeonja-tang, there were nither dead animals nor significant changes of body weights. In addition, no differences were found between control and treated groups in clinical sign and autopsy. 4. Conclusion The data confirmed that Cheongsimyeonja-tang is free from the toxicity and safety problems in treated groups. Compared with the control group, we could not find any toxic alteration in all treated groups(2560, 3200, 4000 and 5000 ㎎/㎏). Lethal Dose 50 (LD50) value for mice was more than 5000 mg/kg per oral for both male and females. It suggest that Cheongsimyeonja-tang in mice is considered to be safe.

Abstract

1. Objectives The aim of this study is data analysis for acute toxicity and safety of Cheongsimyeonja-tang. 2. Methods We investigated the acute toxicity for water-extracted Cheongsimyeonja-tang. Fifty five male and female mice were observed for 14 days after one day oral administration of Cheongsimyeonja-tang at the respective doses of 0 (control group), 2560, 3200, 4000 and 5000 ㎎/㎏. 3. Results We observed survival rates, general toxicity, change of body weight and autopsy. In animals administered with Cheongsimyeonja-tang, there were nither dead animals nor significant changes of body weights. In addition, no differences were found between control and treated groups in clinical sign and autopsy. 4. Conclusion The data confirmed that Cheongsimyeonja-tang is free from the toxicity and safety problems in treated groups. Compared with the control group, we could not find any toxic alteration in all treated groups(2560, 3200, 4000 and 5000 ㎎/㎏). Lethal Dose 50 (LD50) value for mice was more than 5000 mg/kg per oral for both male and females. It suggest that Cheongsimyeonja-tang in mice is considered to be safe.

참고문헌 (18)

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