Mouse Single Oral Dose Toxicity Test of Chongmyung-tang Aqueous Extracts
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Objectives & Methods : The objective of this study was to evaluate the single oral dose toxicity of Chongmyung-tang (CMT) in ICR mice. Korean traditional herbal prescription CMT has traditionally been used as a neuroprotective for treatment of learning disability and memory improvement. CMT, lyophilized aqueous extracts (yield=9.7%) were administered to female and male mice with oral dose of 2,000, 1,000 and 500 mg/kg (body weight) according to the recommendation of Korea Food and Drug Administration (KFDA) Guidelines. Animals were monitored for mortality, changes in body weight, clinical signs and gross observation during 14 days after administration upon necropsy; organ weight and histopathology of 14 principle organs were examined. Results : We could not find any CMT extracts treatment related mortalities, clinical signs, changes in body and organ weight, or gross and histopathological observations against 14 principle organs up to 2,000 mg/kg in both female and male mice, except for some accidental sporadic findings which did not show any obvious dose-relations and most of which also demonstrated in both the female and male vehicle control mice in this experiments. Conclusions : Based on the results of this experiment, the 50% lethal dose (LD50) and approximate lethal dose (ALD) of CMT extracts after single oral treatment in female and male mice can be considered to be over 2,000 mg/kg, and is likely to be safe in humans.
Abstract
Objectives & Methods : The objective of this study was to evaluate the single oral dose toxicity of Chongmyung-tang (CMT) in ICR mice. Korean traditional herbal prescription CMT has traditionally been used as a neuroprotective for treatment of learning disability and memory improvement. CMT, lyophilized aqueous extracts (yield=9.7%) were administered to female and male mice with oral dose of 2,000, 1,000 and 500 mg/kg (body weight) according to the recommendation of Korea Food and Drug Administration (KFDA) Guidelines. Animals were monitored for mortality, changes in body weight, clinical signs and gross observation during 14 days after administration upon necropsy; organ weight and histopathology of 14 principle organs were examined. Results : We could not find any CMT extracts treatment related mortalities, clinical signs, changes in body and organ weight, or gross and histopathological observations against 14 principle organs up to 2,000 mg/kg in both female and male mice, except for some accidental sporadic findings which did not show any obvious dose-relations and most of which also demonstrated in both the female and male vehicle control mice in this experiments. Conclusions : Based on the results of this experiment, the 50% lethal dose (LD50) and approximate lethal dose (ALD) of CMT extracts after single oral treatment in female and male mice can be considered to be over 2,000 mg/kg, and is likely to be safe in humans.
참고문헌 (32)
- Lee MR, "Anti-amnesic effect of chong- myung-tang on scopolamine-induced memory impairments in mice", J Ethnopharmacol, 132(1), pp.70~74 (2010)
- Gōngtíngxián, "Zhòngxìngxiānfāng", pp.36~ (1994), Euiseongdang
- Kim HM, "Inhibition of lipopolysaccharide plus substance P-induced tumor necrosis factor-alpha production from astrocytes by chongmyung-tang", J Ethnopharmacol, 66(3), pp.295~300 (1999)
- Jang KJ, "Chongmyungtang attenuates kainic acid-induced seizure and mortal effect in the mouse", Arch Pharm Res, 20(4), pp.375~378 (1997)
- 오영진, "聰明湯과 香附子聰明湯의 추출물, 나노분말 제형을 이용한 치매에 관한 연구", 동의신경정신과학회지, 17(1), pp.79~105 (2006), 대한한방신경정신과학회
- 박지운, "聰明湯과 木槿皮聰明湯이 CT105와 βA로 유도된 Alzheimer's Disease 病態 모델에 미치는 영향", 동의신경정신과학회지, 17(1), pp.37~57 (2006), 대한한방신경정신과학회
- 임정화, "In vitro에서 β-site amyloid precursor protein-cleaving enzyme 활성과 amyloid β protein 생산에 대한 聰明湯加味方의 효과", 동의신경정신과학회지, 21(2), pp.191~200 (2010), 대한한방신경정신과학회
- 이미라, "Characterization of Korean Traditional Medicine Chongmyungtang for Cognitive Function Related to Anti-cholinesterases and Antioxidant Activity", Food Science and Biotechnology, 20(5), pp.1331~1336 (2011), 한국식품과학회
- Korea Food and Drug Administration, "Testing Guidelines for Safety Evaluation of Drugs", Korea Food and Drug Administration, ~ (2009)
- Organization for Economic Co-Operation and Development, "OECD guideline (423) for testing of chemicals-acute oral toxicity-acute toxic class method", ~ (2001)
- Irwin S, "Comprehensive observational assessment: Ia. A systematic, quantitative procedure for assessing the behavioral and physiologic state of the mouse", Psychopharmacologia, 13(3), pp.222~257 (1968)
- JaeHyun Lee, "Single Subcutaneous Dose Toxicity of Polycan？, a β-Glucan Originated from Aureobasidium in Mice", Laboratory Animal Research, 21(4), pp.299~305 (2005), 한국실험동물학회
- Hyeung-Sik Lee, "Single Oral Dose Toxicity Studies of Polycan, b-Glucan Originated from Aureobasidium in Mice", 한국독성학회지, 21(4), pp.361~365 (2005), 한국독성학회
- Hyeung-Sik Lee, "Single Oral Dose Toxicity Study of Water Extractsof Picrorrhiza Rhizoma In ICR Mice", 한국독성학회지, 22(2), pp.117~126 (2006), 한국독성학회
- 노성수, "Mouse Single Oral Dose Toxicity Study of DHU001, a Polyherbal Formula", 한국독성학회지, 26(1), pp.53~59 (2010), 한국독성학회
- Levene A, "Pathological factors influencing excision of tumors in the head and neck. Part 1", pp.145~151 (1981), Clin Otalary
- Ludbrook J, "Microcomputer statistics packages", A personal view. Clin Exp Pharmacol Physiol, 24, pp.294~296 (1997)
- Jin BY PJ, "Studies on the alkaloidal components of polygala tenuifolia willd", Zhongguo Zhong Yao Za Zhi, 18, pp.675~677 (1993)
- Sun X, "Studies on chemical constituents of fat oil of polygala tenuifolia", Zhong Yao Cai, 23(1), pp.35~ (2000)
- Jiang Y, "Xanthone O-glycosides from polygala tenuifolia", Phytochemistry, 60(8), pp.813~816 (2002)
- Kim H, "Screening and isolation of antibiotic resistance inhibitors from herb materials IV- resistance inhibitors from anetheum graveolens and acorns gramineus", Arch Pharm Res, 21(6), pp.734~737 (1998)
- Zhu YP, "Chinese Material Medica Chemistry, Pharmacology and Applications", pp.541~543 (1998), Harwood Academic Publishers
- Luo L, "Antidepressant effects of banxia houpu decoction, a traditional chinese medicinal empirical formula", J Ethnopharmacol, 73, pp.277~281 (2000)
- Kim YK, "Comparative effect of Prunus persica L. BATSCH-water extract and tacrine (9-amino- 1,2,3,4-tetrahydroacridine hydrochloride) on concentration of extracellular acetylcholine in the rat hippocampus", J Ethnopharmacol, 87, pp.149~154 (2003)
- Kulkarni PV, "Quinoline-n- butylcyanoacrylate-based nanoparticles for brain targeting for the diagnosis of Alzheimer's disease", Wiley Interdiscip Rev Nanomed Nanobiotechnol, 2(1), pp.35~47 (2010)
- Allegri RF, "Cerebrolysin improves symptoms and delays progression in patients with Alzheimer's disease and vascular dementia", Drugs Today, 48(Suppl A), pp.25~41 (2012)
- Corbett A, "New and emerging treatments for Alzheimer's disease", Expert Rev Neurother, 12, pp.535~543 (2012)
- Flecknell P, "Laboratory Animal Anesthesia", pp.269~ (1996), Harcourt Brace&Company
- Plata EJ MW, "Growth and hematologic properties of the BALB", wm strain of inbred mice, 1972, pp.22, 712~720
- Yamauchi C, "Effects of room temperature on reproduction, body and organ weight, food and water intakes, and hematology in mice", Jikken Dobutsu, 32(1), pp.1~11 (1983)
- Banks WJ, "Remale reproductive system in Applied veterinary histology", pp.505~526 (1986), Williams&Wilkins
- Pineda MH, "Female reproductive system in Veterinary endocrinology and reproduction", pp.303~354 (1989), Lea&Febiger