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水火旣濟湯 증류액이 노화 흰쥐의 비장세포 면역활성에 미치는 영향

The Effect of Suhwagije-tang Distillate on the Immune Activity of Spleen Cells of Aged Rats

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1. Objectives The purpose of this study is to investigate the effect of Suhwagije-tang(SGT) distillate on the immune activity of spleen cells of aged SD rats. 2. Methods We used 10, 50, 72 weeks old SD rats in this study. Spleen cells from SD rats were stimulated with ConA and treated with 1% Vitamin C(Vit.C) or Suhwagijetang distillate(SGT). After 24 hours, the concentrations of IL-2, IL-4,IL-10, IFN-γ in the cell culture supernatant were measured by ELISA. 3. Results and Conclusions 1) At all concentration of SGT distillate, survival rates of liver cells were higher than the control group. In addition,50% SGT distillate group's cell survival rates were significantly higher than other groups. 2) In 10 weeks SD rats(SGT group), the concentration of IL-2 significantly decreased in comparison with ConA group, Vit.C group. In 52 weeks SD rats(SGT group), the concentration of IL-2 significantly decreased in comparison with ConA group. 3) In 10, 52 weeks SD rats(SGT group), the concentration of IL-4 significantly decreased in comparison with ConA group. 4) In 10 weeks SD rats(SGT group), the concentration of IL-10 significantly decreased in comparison with ConA group. And in 72 weeks SD rats(SGT group), the concentration of IL-10 significantly increased in comparison with Vit.C group. 5) In 52, 72 weeks SD rats(SGT group), the concentration of IFN-γ significantly decreased in comparison with 10 weeks SD rats(SGT group). These results suggest that Suhwagije-tang(SGT) distillate has the effect of increasing the immune activity of spleen cells of aged SD rats.

Abstract

1. Objectives The purpose of this study is to investigate the effect of Suhwagije-tang(SGT) distillate on the immune activity of spleen cells of aged SD rats. 2. Methods We used 10, 50, 72 weeks old SD rats in this study. Spleen cells from SD rats were stimulated with ConA and treated with 1% Vitamin C(Vit.C) or Suhwagijetang distillate(SGT). After 24 hours, the concentrations of IL-2, IL-4,IL-10, IFN-γ in the cell culture supernatant were measured by ELISA. 3. Results and Conclusions 1) At all concentration of SGT distillate, survival rates of liver cells were higher than the control group. In addition,50% SGT distillate group's cell survival rates were significantly higher than other groups. 2) In 10 weeks SD rats(SGT group), the concentration of IL-2 significantly decreased in comparison with ConA group, Vit.C group. In 52 weeks SD rats(SGT group), the concentration of IL-2 significantly decreased in comparison with ConA group. 3) In 10, 52 weeks SD rats(SGT group), the concentration of IL-4 significantly decreased in comparison with ConA group. 4) In 10 weeks SD rats(SGT group), the concentration of IL-10 significantly decreased in comparison with ConA group. And in 72 weeks SD rats(SGT group), the concentration of IL-10 significantly increased in comparison with Vit.C group. 5) In 52, 72 weeks SD rats(SGT group), the concentration of IFN-γ significantly decreased in comparison with 10 weeks SD rats(SGT group). These results suggest that Suhwagije-tang(SGT) distillate has the effect of increasing the immune activity of spleen cells of aged SD rats.

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