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生薑甘草湯 및 生薑, 甘草가 생쥐 비장세포의 활성에 미치는 영향

Effects of Senggang Gamcho-tang (SG), ZingberisRhizoma (ZR) and Glycyrrihizae Radix (GR) extracts in Senggang Gamcho-tang (SG) on Activity of Murine Splenocytes

김진주|정희재|정승기|이매
대한한방내과학회 (The Society Of Korean Oriental Internal Medicine)|2011.09|대한한방내과학회지 (The Journal of Korean Oriental Internal Medicine)|제32권 제3호|pp. 323-333

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키워드

allergycytokineIL-4IFN-γSenggang Gamcho-tang (Shengjianggancao-tang)Zingberis RhizomaGlycyrrihizae Radix
주제분류한의학

초록

Objectives : Zingberis Rhizoma (ZR) and Glycyrrihizae Radix (GR) have been widely used to prevent or treat allergic diseases. However, relatively little research has been conducted on the immune response to Senggang Gamcho-tang (SG) in a mixture of ZR and GR. The purpose of this study was to determine antiallergic effects of Senggang Gamcho-tang (SG) extracts ZR and GR on activity of murine splenocytes. Methods : This study was performed to investigate the effect of SG in mice, using in vitro experiments. Cells were treated with SG extract (1 µg/ml, 10 µg/ml and 100 µg/ml) plus Con A (2 µg/ml) and ZR (6.6 µg/ml and 66 µg/ml), GR (3.3 µg/ml and 33 µg/ml) respectively for 48 hrs. The production of IFN-γ and IL-4 were determined by ELISA. Results : No toxicity was found in splenocytes treated with SG extract for 48 hrs at the concentration of 0 µg/ml, 1 µg/ml, 10 µg/ml and 100 µg/ml, respectively. The production of IFN-γ increased to 23,968.7 pg/ml (p<0.001) while that of IL-4 significantly decreased to 14.1 pg/m l(p<0.05) only at the SG concentration of 100 µg/ml. With the GR concentration of 33 μg/ml in SG extract, the production of IFN-γ rose to 32,102.2 pg/ml (p<0.001) while that of IL-4 (p<0.05) declined to 34.2 pg/ml. However, there was no significant observation with regard to the effect of ZR on production of IFN-γ and IL-4. Conclusions : This study suggests an antiallergic effect of Senggang Gamcho-tang, which leads to the implication that SG extract may be used to enhance human immune function.

Abstract

Objectives : Zingberis Rhizoma (ZR) and Glycyrrihizae Radix (GR) have been widely used to prevent or treat allergic diseases. However, relatively little research has been conducted on the immune response to Senggang Gamcho-tang (SG) in a mixture of ZR and GR. The purpose of this study was to determine antiallergic effects of Senggang Gamcho-tang (SG) extracts ZR and GR on activity of murine splenocytes. Methods : This study was performed to investigate the effect of SG in mice, using in vitro experiments. Cells were treated with SG extract (1 µg/ml, 10 µg/ml and 100 µg/ml) plus Con A (2 µg/ml) and ZR (6.6 µg/ml and 66 µg/ml), GR (3.3 µg/ml and 33 µg/ml) respectively for 48 hrs. The production of IFN-γ and IL-4 were determined by ELISA. Results : No toxicity was found in splenocytes treated with SG extract for 48 hrs at the concentration of 0 µg/ml, 1 µg/ml, 10 µg/ml and 100 µg/ml, respectively. The production of IFN-γ increased to 23,968.7 pg/ml (p<0.001) while that of IL-4 significantly decreased to 14.1 pg/m l(p<0.05) only at the SG concentration of 100 µg/ml. With the GR concentration of 33 μg/ml in SG extract, the production of IFN-γ rose to 32,102.2 pg/ml (p<0.001) while that of IL-4 (p<0.05) declined to 34.2 pg/ml. However, there was no significant observation with regard to the effect of ZR on production of IFN-γ and IL-4. Conclusions : This study suggests an antiallergic effect of Senggang Gamcho-tang, which leads to the implication that SG extract may be used to enhance human immune function.

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