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龍肉調胃湯 경구투여가 노화 흰쥐의 면역활성에 미치는 영향

The Effect of Oral Administration of Yongyukjowi-tang on the Immune Activity in Aged Rat

안택원|이한얼
사상체질면역의학회 (The Society of Sasang Constitution and Immune Medicine)|2012.12|사상체질면역의학회지 (Journal of Sasang Constitution and Immune Medicine)|제24권 제4호|pp. 62-74|DOI http://dx.doi.org/10.7730/JSCM.2012.24.4.62

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키워드

Immune cellCytokineImmune activityYongyukjowi-tangAged Rat
주제분류한의학

초록

Objectives The purpose of this study is to investigate the effect of oral administration of Yongyukjowi-tang(YJT) on the immune activity in aged Sprague-Dawley rat(SD rat). Methods SD rats were divided into three groups: Yongyukjowi-tang groups(YJT groups), distilled water groups(control groups) and Vitamin C groups(positive control groups) which were administered an oral dose(1㎖/1day) to 6, 48 and 68 weeks old SD rats for four weeks. After four weeks, the number of total leukocyte, CD3+, CD4+, CD8+, and the level of cytokine (IL-2, IL-4, IL-10, IFN-γ) were measured in spleen tissue of each SD rats. Results and Conclusions 1) The number of total leukocyte significantly increased in 52 weeks old YJT group and 72 weeks old YJT group in comparison with those of the control group. 2) The number of CD3+ cell significantly increased in 72 weeks old YJT group in comparison with those of the control group and the positive control group. 3) The number of CD8+ cell significantly increased in 52 weeks old YJT group in comparison with those of the control group. 4) The level of IL-2 significantly increased in 72 weeks old YJT group in comparison with those of the control group and the positive control group. 5) The level of IL-4 significantly increased in 52 weeks old YJT group in comparison with those of the positive control group. These results suggest that oral administration of YJT has an effect on increase of immune activity in aged rat.

Abstract

Objectives The purpose of this study is to investigate the effect of oral administration of Yongyukjowi-tang(YJT) on the immune activity in aged Sprague-Dawley rat(SD rat). Methods SD rats were divided into three groups: Yongyukjowi-tang groups(YJT groups), distilled water groups(control groups) and Vitamin C groups(positive control groups) which were administered an oral dose(1㎖/1day) to 6, 48 and 68 weeks old SD rats for four weeks. After four weeks, the number of total leukocyte, CD3+, CD4+, CD8+, and the level of cytokine (IL-2, IL-4, IL-10, IFN-γ) were measured in spleen tissue of each SD rats. Results and Conclusions 1) The number of total leukocyte significantly increased in 52 weeks old YJT group and 72 weeks old YJT group in comparison with those of the control group. 2) The number of CD3+ cell significantly increased in 72 weeks old YJT group in comparison with those of the control group and the positive control group. 3) The number of CD8+ cell significantly increased in 52 weeks old YJT group in comparison with those of the control group. 4) The level of IL-2 significantly increased in 72 weeks old YJT group in comparison with those of the control group and the positive control group. 5) The level of IL-4 significantly increased in 52 weeks old YJT group in comparison with those of the positive control group. These results suggest that oral administration of YJT has an effect on increase of immune activity in aged rat.

참고문헌 (39)

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