닭에서 Camphylobacter jejuni/ coli의 분리빈도와 시험관내 항균력

Abstract

Campylobacter jejuni/ coli have been reconignized as important agents of gastroenteritis throughout the world. C. jejuni infection in Korea has been reporeted in 1981, but the epidemiological information was laking in the report. This study was undertaken to determine the Campylobacter carrier rate of the chickens in Chullabuk - Do. To determine monthly the Campylobacter carrier rate of chickens, cultures were made from 313 samples taken in Iksan city, Chullabuk-Do during January 1996 to August 1996. Also, to find out susceptibility of 13 antimicrobial agents against 30 isolates of C. jejuni/ coli from chickens were determined by the disk diffusion and the agar dilution technique. The following results were obtained. First, C. jejuni/ coli was isolated from 64 % of chicken samples, and the frequency of isolation was highest in August, while the lowest 11 % in April. The seasonal vari ation of the rate was not remarkable. Second, all of the isolates from chickens were susceptible to amikacin, gentamicin, imipenem and tobramycin. To erythromycin 12 %, nalidixic acid 22%, ciprofloxacin 70% and tetracycline 59% of isolates showed resistance. Imipenem, gentamicin, tobramycin and amikacin were the low antibiotic agents with MIC90 of 0.25, 1, 2, and 2㎍/ml， respectively. Imipenem was the most antimicrobial agent tested, and MIC range of that was 0.06~0.25㎍/ml and MIC50 and MIC90 were 0.12㎍/ml and 0.25㎍/ml, respectively. Third, when the suceptibility test by the disk diffusin and by the agar dilution method were tested, resistance to ampicillin and tetracycline were 21 % and 40% respectively, but no difference were noted against other antibiotics. In conclusion, they remains susceptible to aminoglycoside and imipenem.