Prevalence of virulence-associated genes and antimicrobial resistance of Campylobacter jejuni from ducks in Gyeongnam Province, Korea
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Total 99 strains of Campylobacter spp. were isolated from 117 cases of duck’s fecal samples. Among 99 strains of Campylobacter spp. isolates, 93 strains (93.9%) were C. jejuni and 6 strains (6.1%) were C. coli. Prevalence of virulence and GBS associated genes of 72 C. jejuni isolates was determined by m-PCR. Among the 10 kinds of virulence associated genes, cadF, dnaJ, flaA and ceuE genes were de-tected in all of C. jejuni isolates from ducks, racR, pldA, iamA, ciaB, virB11 and docC genes were 87.5%, 84.7%, 77.8%, 48.6%, 13.9% and 11.1%, respectively. Antimicrobial susceptibility test was per-formed on 72 C. jejuni isolates. The rate of resistance were 62.5% for oxytetracycline, 55.6% for kana-mycin, 54.2% for enrofloxacin, 50% for ciprofloxacin, 37.5% for tetracycline and nalidixic acid, 18.1% for ampicillin, 15.3% for streptomycin, and 6.9% for ofloxacin. All isolates were susceptible to erythromycin. The adherence (intracellular and extracellular bacteria) abilities of the 20 isolates to INT-407 cells were between 4.21±1.27×104 CFU/well and 1.053±0.451×106 CFU/well from the isolates of cj-55 and cj-52, respectively, and that can be expressed as 0.1033% to 5.2655% to the infecting inoculum. The invasion (intracellular bacteria) abilities of the 20 isolates to INT-407 were between 1.00±1.73×103 CFU/well and 8.47±5.16×104 CFU/well from the isolates of cj-13 and cj-47, respectively, and that can be expressed as 0.0050% to 0.4235% to the infecting inoculums. The average CFU/well of 20 campylobacters isolated from ducks for adherence to and invasion were 2.646±2.886×105 and 3.03±2.7×104 respectively, and that was 1.3230±1.2139% and 0.1516±0.1343% of the starting viable inoculum. There was considerable correlation (R2=0.627) between the adherence and invasion ability of C. jejuni isolates for INT-407 cell.
Abstract
Total 99 strains of Campylobacter spp. were isolated from 117 cases of duck’s fecal samples. Among 99 strains of Campylobacter spp. isolates, 93 strains (93.9%) were C. jejuni and 6 strains (6.1%) were C. coli. Prevalence of virulence and GBS associated genes of 72 C. jejuni isolates was determined by m-PCR. Among the 10 kinds of virulence associated genes, cadF, dnaJ, flaA and ceuE genes were de-tected in all of C. jejuni isolates from ducks, racR, pldA, iamA, ciaB, virB11 and docC genes were 87.5%, 84.7%, 77.8%, 48.6%, 13.9% and 11.1%, respectively. Antimicrobial susceptibility test was per-formed on 72 C. jejuni isolates. The rate of resistance were 62.5% for oxytetracycline, 55.6% for kana-mycin, 54.2% for enrofloxacin, 50% for ciprofloxacin, 37.5% for tetracycline and nalidixic acid, 18.1% for ampicillin, 15.3% for streptomycin, and 6.9% for ofloxacin. All isolates were susceptible to erythromycin. The adherence (intracellular and extracellular bacteria) abilities of the 20 isolates to INT-407 cells were between 4.21±1.27×104 CFU/well and 1.053±0.451×106 CFU/well from the isolates of cj-55 and cj-52, respectively, and that can be expressed as 0.1033% to 5.2655% to the infecting inoculum. The invasion (intracellular bacteria) abilities of the 20 isolates to INT-407 were between 1.00±1.73×103 CFU/well and 8.47±5.16×104 CFU/well from the isolates of cj-13 and cj-47, respectively, and that can be expressed as 0.0050% to 0.4235% to the infecting inoculums. The average CFU/well of 20 campylobacters isolated from ducks for adherence to and invasion were 2.646±2.886×105 and 3.03±2.7×104 respectively, and that was 1.3230±1.2139% and 0.1516±0.1343% of the starting viable inoculum. There was considerable correlation (R2=0.627) between the adherence and invasion ability of C. jejuni isolates for INT-407 cell.
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