퀴롤논 내성 Campylobacter jejuni의 분리와 항균제 감수성

Abstract

Campylobacter spp. are frequent cause of enteric infecton. Increased resistance of human isolates of Campylobacter was considered in part due to widespread animal use of antimicrobial agents. In Korea, our knowledge on both human and animal carriage of Campylobacter is very limited. In this study, 260 stool specimens were taken from healthy subjects in Soonchun and Iksan city, and intestinal contents of 231 chickens during the period between August 1998 to July 1999. The Isolaton rate of Campylobacter from healthy subjects was 1.9%, and the rate was higher in ≤10-year-old children. The isolation rate from healthy chicken was 66.3%, and the rate was highest in August (80%) and lowest in December (56.5%). All of the C. jejuni from chicken were susceptible to amikacin, clindamycin and gentamicin. The resistance rates were 97% to tetracycline, 82% to ciprofloxacin,nalidixic acid and co-trimoxazloe, 65% to ampicillin. All of the C. jejuni isolates from human were resistant to ampicillin and co-trimoxazole, while the rates were 67% for both ciprofloxacin and tetracycline. For C. jejuni, antimicrobial agents with lower MICs were imipenem ≤0.06 μg⁄ml, gentamicin 0.25 μg⁄ml, amikacin 0.5-4 μg⁄ml and kanamycin 2-8μg⁄ml. MIC ranges of ciprofloxacin and nalidixic acid were 0.06-64 μg⁄ml and 32-64 μg⁄ml, respectively. Susceptibilities to nalidixic acid and to ciprofloxacin were identical Ampicillin and tetracycline resistance rates were higher among nalidixic acid-resistant isolates than susceptible ones. In conclusion, human carriage rate of Campylobacter is very low, while the rate in chickens is very high. Fluoroquinolone resistance rate of C. jejuni is very high, but amikacin and gentamicin can be used empirically without susceptibility testing as resistance to these antibiotics seem to be rare.