일밤 파라핀 절편에서 wet autoclave 전처리 후 AgNORs의 검출

Abstract

In the last few years the AgNORs technique has been used in tumor pathology for both diagnostic and prognostic patposes, but the lack of a standardizied staining protocol has frequently let to misinterpretation of actual structures evaluated by the various authors. The aim of the present study set out to measure the effect of wet autoclave pretreatment of routinely form alin -fixed and paraffin -embedded tissues on AgNORs staining. AgNOR quantity was evaluated n archival tumor tissues of 5 invasive adenocarcinoma of stomach , 8 invasive ductal carcinoma of breast and 14 invasive squamous cell carcinoma of uterine cervix. The treatment of sections at superheatedsteam temperature(121 degrees C) before silver incubation leads to a significantly better discrimination of individu a1 AgNORs , especially in AgNOR aggregates of nucleolus.Counting "black dots" by eye revealed considerably higher AgNOR mean numbers pernucleus in all pretreated cases when compared with the respective untreated controls. Also, mean AgNOR numbers increased after wet autoclave pretreatment in a linear manner(pairwise comparison by means of Pearson coπelation coefficient: r=0.89 instomach cancer, r=0.86 in breast cancer, r=0.86 in cervix cancer). It is suggested that the wet autoclave-pretreated method in routinely formalin-fixed and paraffin-embedded archival materials is more sensitive in detecting NORs than the untreated AgNORs staining and may prove a useful altemative for application in tumor pathology.