소절편 조직의 신속보고 체계에 대한 연구 -새로운 조직 고정액과 표본제작-

Abstract

Generally and traditionally surgical specimens are fixed in 10% buffered formalin at room temperature. But when we used 10% buffered formalin for tissue fixatives, Toxic gas and vapors are take plase in laboratory. By this toxic gas and we can't breathing and working for long time, and more, tissue fixation procedires require various amount of reagents and time depending on speciemn size and type. So, we introduced new equipment and fixatives such as Microwave and Histochoice-formalin. Microwave technology can be used to enhance specimen fixation by promoting chemical bonding and penetration of reagents into tissues. As such microwaves have been used to decrease the time of tissue fixation. This study was undertaken to develop a rapid universal fixation procedure using a low concentration of formalin and microwaves technology. Microwave oven (Pelco, 3440, 700W) was used for fixation, and this made monotpring and controlling of time and feasible temperature. A various tissue specimens such as brain, colon, lung, spleen, stomach and uterus were in 3% formalin in Histochoice (Ameresco, Solon, OH). The specimens were processsed at a varoius condition of powers, times and temperature in the special tissue processor that have vaccum function (Pathentre, Shadon, USA). A comparative study for 10% neutral buffered formalin with or without microwave irradiation was done. We performed H&E stain, histochemistry, immunohistochemistry, and insitu hydridization using the rapidly fixed sections and compared with routinely processed tissue sections. The optomal temperature nad irradiation timefor suitable fixation was determined to be 60±3℃ at 300±30 sec at 700 W. The optimal concentration for tissue fixatives is 3% Histochoice-formalin, which composed of Histochice-tissue fixative ; 200 ml, Sodium acetate ; 3 gm, Sodium hydroxide ; 8 gm, Sodium phosphate monobasic l 10.5 gm, Sodium phosphate dibasic ; 19.5 gm, 40% Formalin. The rapid fixaton in 3 % formalin in Histochoice by microwave has similar quality and sensitivity in H&E, histochemistry，immunohistochemistry and in situ hybridization, compared with 10% buffered formalin with or without irradiation. Total consumed time for tissue process, focus on small specimen, is 1h 37 min. Conclusion : The fixation time was signiftcantly reduced, and rapid pathological diagnosis could be performed. The sensitivity for immunohistochemistry was qualitatively increased. The environment of gross cutting room was comfortable due to the low formalin concentration. This new method was very useful tools with simple and rapid for tissue processing and pathologic diagnosis.