면역세포화학적 염색을 위한 고정제의 비교 평가

Abstract

Immunocytochemical staining is now a important tool for tumor typing in diagnostic cytology. However it is less used than immunocytochemical staining. This may be due to methodologic difficuties. Therefore, this study compared few. fixative methods for immunocytochemical staining in diagnostic cytology. Three commonly used fixative methods were evaluated: (1) 0.05% glutaraldehyde at RT forlmin or 15min; (2) 95 % alcohol at RT for 30min, and at 4ºC for overnight; (3) acetone/alcohol mixture at RT 30min and 4ºC for overnight. Specimens were obtained from colon adenocarcinoma and lymph nodes. Immunocytochemical staining was performed with antibodies directed to 3 different types of antigens: (1) antibodies directed to the imtermediate filament proteins, cytokeratin and vimentin;(2) antibodies directed to the cytoplasmic proteins (lymphoreticular system markers), leukocyte common antigens (LCA); (3) and antibodies directed to the nocofetal antigens (carcino embryonic antigen; CEA). The reults showed that immunocytochemical staining on the specimens fixed with 0.05% glutaraldehyde at RT was better than those with other fixative methods.