Papanicolaou stain의 시간단축 및 시약절약을 위한 세포질 염색시약의 연구
The Study of Cytoplasm Stain Reagents for Saving Up Time and Reagents in Papanicolaou Stain
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Abstract
Currently, Orange-G and EA50 are being used in Papanicolaou stain. The simplification of this two reagents is studied in this research. According to the result, the most favorable arrangement condition is like the following. 1) It is proved that the most satisfactory stain property is made when Orange-G is 2.5ml, Light green is 4.5ml and Eosin-Y is 7.0ml in the aspect of density. 2) The best qulified stain property is made up when the pH is 5.0∼5.5 in the aspect of pH in mixed reagent. Therefore, for the optimum mixture of reagents, you should mix Orange-G 2.5ml, Light geen 4.5ml and Eosin-Y 7.아nl together, and then, add alcohol whose alcoholicity is 95 percent to it when it comes to the amount of 1000ml. And you should add phosphotungstic acid 2g, saturated solution of lithium carbonate 0.5ml and glacial acetic acid to it in a row until it comes to pH5.0-5.5.