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이미페넴에 내성을 가진 녹농균의 Class 1 Integron의 분석

Analysis of Class 1 Integrons in Imipenem-resistant Pseudomonas aeruginosa

성지연
대한임상검사과학회 (Korean Society for Clinical Laboratory Science)|2011.06|대한임상검사과학회지 (Korean Journal of Clinical Laboratory Science)|제43권 제2호|pp. 68-74

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Abstract

Pseudomonas aeruginosa is an aerobic, Gram-negative, glucose-nonfermenting bacterium, which has emerged as a serious opportunistic pathogen. Recently, outbreaks of carbapenem resistant P. aeruginosa give rise to significant therapeutic challenges for treating nosocomial infections. The genes of metalloß- lactamase (MBL), a powerful carbapenemase, are carried as a part of the mobile gene cassettes inserted into integrons playing an important role in rapid dissemination of antibiotic resistance genes among bacterial isolates. In this study, we investigated the prevalence of integron in imipenem resistant P. aeruginosa isolates. A total of 61 consecutive, non-duplicate, and imipenem resistant P. aeruginosa strains were isolated from a university hospital in the Chungcheong province of Korea. We employed repetitive extragenic palindromic sequence-based PCR (rep-PCR) method for the selection of clonally different P. aerusinosa strains. PCR and DNA sequencing were conducted for the detection of integrons. Twenty-one clonally different P. aeruginosa strains were isolated. Only one (P28) of the strains harbored blaVIM-2 that was found as gene cassettes in class 1 integrons. Four of 21 carbapenem resistant P. aeruginosa strains harbored class 1 integron containing aminoglycoside resistance determinant. All of the integrons detected in the study contained more than one resistance gene cassette, which can mediate resistance to multiple antibiotics. To prevent further spreading of the multi-drug resistant P. aeruginosa, conseguent monitoring and clinical polices are required.

참고문헌 (26)

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Metallo-β-lactamase 생성 녹농균에서 Class 1 Integron의 유전형 분석고지선, 구선회, 권계철, 박종우, 성지연, 송정훈, 신소연대한임상미생물학회 · Annals of Clinical Microbiology2009 · 제12권 제1호금속성 Lactamase를 생성하는 녹농균의 표현형과 유전형 탐지권영훈, 박미선, 양병선, 이민주, 이혜인, 정승봉, 정종윤, 최승구, 홍근석대한임상검사과학회 · 대한임상검사과학회지2012 · 제44권 제2호Pulsed-Field Gel Electrophoresis 를 이용한 원내 감염균 Pseudomonas aeruginosa의 역학분석을 위한 유전형 조사김봉철대한임상검사과학회 · 대한임상검사과학회지1996 · 제28권 제1호Characteristics of Acquired β-lactamase Gene in Clinical Isolates of Multidrug-resistant Pseudomonas aeruginosa고지선, 구선회, 권계철, 박종우, 성지연, 송정훈, 신소연, 정선양대한임상미생물학회 · Annals of Clinical Microbiology2008 · 제11권 제2호접합 가능한 다제내성 녹농균의 출현이미영한국미생물·생명공학회 · 한국미생물·생명공학회지2023 · 제51권 제4호대구지역병원에서 분리된 Acinetobacter baumannii와 Pseudomonas aeruginosa의 항생제 내성현황김수정, 이재식대한임상검사과학회 · 대한임상검사과학회지2008 · 제40권 제2호

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