금속성 Lactamase를 생성하는 녹농균의 표현형과 유전형 탐지
Phenotypic and Genotypic Detection of Metallo-ß-Lactamase Producing Pseudomonas aeruginosa
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Abstract
This study was undertaken to evaluate phenotypic and genotypic methods for detection of Metallo- Beta-Lactamases (MBLs) among nosocomial Pseudomonas aeruginosa. Of the 50 P. aeruginosa isolates from clinical specimens, 20 were evaluated for carbapenem resistance and screened for MBL by doubledisk synergy test and combined-disk test. Nineteen strains (95%) were found to be MBL producers among the 20 P. aeruginosa. MBL positives were further confirmed by Polymerase Chain Reaction (PCR). For the IMP and VIM types of MBLs, PCR analysis was performed on 19 of the 20, and 10 were positive for VIM MBL type. This study reports the validation of a simple and accurate MBL detection method that can be easily incorporated into the daily routine of a clinical laboratory. Early detection of MBL-carrying organisms, including those with susceptibility to carbapenems, is of paramount clinical importance, as it allows rapid initiation of strict infection control practices as well as therapeutic guidance for confirmed infection.Key Words : Hepatitis A virus (HAV), Anti-HAV, Hospital workers, Prevalence, Vaccination.
참고문헌 (10)
- Behera B, "An evaluation of four different phenotypic techniques for detection of metallo-ß-lactamase producing Pseudomonas aeruginosa", Indian J Med Microbiol, 26, pp.233~237 (2008)
- Bush K, "ß-Lactamases of increasing clinical importance", Curr Pharm Des, pp.839~845 (1999)
- Castanheira M. Bell JM, "Carbapenem Resistance among Pseudomonas aeruginosa Strains from India: Evidence for Nationwide Endemicity od Multiple Metallo-ß-Lactamase Clones (VIM-2, -5, -6, and -11 and the Newly Characterized VIM-18)", Antimicrob Agents Chemother, 53, pp.1225~1227 (2009)
- Cirioni O, "Efficacy of tachyplesinIII, colistin, and imipenem against a multiresistance Pseudomonas aeruginosa Strain", Antimicrob Agents Chemothor, 51, pp.2005~2010 (2007)
- Gales AC, "Dissemination in distinct Brazilian regions of an epidemic carbapenem-resistant Pseudomonas aeruginosa producing SPM Metallo-ß-Lactamase", J Antimicrob Chemother, 52, pp.699~702 (2003)
- Lee K, "VIM- and IMP-type metallo-ß-lactamase-producing Pseudomonas spp. and Acinetobacter spp. in Korean hospitals", Emerg Infect Dis, 9, pp.868~876 (2003)
- Pitout JD, "Detection of Pseudomonas aeruginosa producing metallo-ß-lactamases in a large centralized laboratory", J Clin Microbiol, 43, pp.3129~3135 (2005)
- Tsakris A, "VIM-1 metalloß-lactamase in Acinetobacter baumannii", Emerg infect Dis, 12, pp.981~983 (2006)
- Walsh TR, "Metallo-ßlactamasses:the quiet before the storm?", Clin Microbiol Rev, pp.18306~18325 (2005)
- Yong D, "Imipenem-EDTA disc method for differentiation of metallo-ß-lactamase-producing clinical isolates of Pseudomonas spp. And Acinetobacter spp", J Clin Microbiol, 40, pp.3798~3801 (2002)