치매유발물질인 염화알루미늄으로 손상된 C6 신경교종세포주에서의 항산화 효과
Antioxidative Effect of Aster yomena (Kitm.) Extract on C6 Glioma Cell Line Damaged by AlCl3, Dementia Inducer
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초록
본 연구는 치매유발물질의 하나인 염화알루미늄(AlCl3)의 신경독성을 C6 glioma세포를 배양하여 조사하였으며, AlCl3의독성에 대한 쑥부쟁이[Aster yomena (Kim.), AY] 추출물의 보호효과를 조사하였다. 또한, 세포생존율을 비롯하여 항산화능분석인 XO 저해능과 SAR-소거능을 조사하였다. 그 결과 AlCl3 는 배양 세포에 처리한 농도에 비례적으로 세포생존율을 유의하게 감소시켰다. 이 과정에서 XTT50값이 130.0 μM로 나타나Borenfreund와 Puerner의 독성판정기준에 의하여 중간독성(mid-cytotoxic)으로 나타났다. 한편, 항산화제인 QU는AlCl3의 독성으로 손상된 세포생존율을 유의하게 증가시켰다. 또한, AlCl3의 독성에 대한 AY 추출물의 방어효과에 있어서, AY 추출물은 AlCl3만의 처리에 비하여 세포생존율을 유의하게증가시켰으며, 이와 동시에 XO 저해능과 SAR-소거능과 같은항산화 효과를 나타냈다. 이상의 결과로부터 AlCl3의 독성에 산화적 손상이 관여하고 있으며, AY 추출물은 항산화 효과에 의하여 AlCl3의 독성을 효과적으로 방어하였다. 따라서, AY 추출물과 같은 천연물질은 질환유발제인 AlCl3와 같이 산화적 손상과관련이 있는 중금속화합물의 독성 완화나 질환의 치료적 개선을위한 대체물질개발에 있어 유용한 소재인 것으로 생각된다.
Abstract
This study examined the neuronal cytotoxicity of aluminum chloride (AlCl3), a dementia inducer, and the protective effects of Aster yomena (Kitam.)(AY) extract on AlCl3-induced cytotoxicity in cultured C6 glioma cells. The antioxidative effects, such as the inhibitory ability of xanthine oxidase (XO) and superoxide anion-radical (SAR) scavenging ability, on cell viability were examined. AlCl3 decreased the cell viability significantly in a dose-dependent manner, and the XTT50 value was 130.0 μM in these cultures. The cytotoxicity of AlCl3 was determined to be mid-toxic according to the Borenfreund and Puerner’ toxic criteria. Quercetin (QU), an antioxidant, increased the cell viability reduced by AlCl3-induced cytotoxicity. The protective effect of the AY extract on AlCl3-induced cytotoxicity was analyzed. The AY extract increased the cell viability remarkably compared to the AlCl3-treated group and showed the inhibitory ability of XO and SAR-scavenging ability. The cytotoxicity of AlCl3 was correlated with oxidative stress, and the AY extract effectively prevented AlCl3-induced cytotoxicity through its antioxidative effects. In conclusion, natural resources, such as the AY extract, may be a putative agent for improving the cytotoxicity of heavy metallic compounds correlated with oxidative stress, such as AlCl3, a morbid agent.
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