치매유발제인 알루미늄에 대한 Rosmarinic Acid의 보호 효과
The Protective Effect of Rosmarinic Acid on the Aluminum of Dementia Inducer
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초록
치매유발제인 AlCl3에 대한 rosmarinic acid의 영향을 알아보기 위하여 뇌세포종인 배양 C6 glioma 세포를 배양한 후 세포생존율을 비롯한 전자공여능(electron donating ability, EDA), SOD-유사활성(superoxide dismutase-like activity) 및 지질과산화(lipid peroxidation) 억제능을 조사하였다. 본 연구에서 AlCl3는 배양 세포에 농도 의존적으로 세포생존율을 대조군에 비하여 유의하게 감소시킴으로서 세포독성을 나타냈다. 또한, AlCl3의 XTT50값이 142.2 μM에서 나타남으로 이는 Borenfreund와 Puerner의 독성판정기준에 의하여 중간독성(mid-toxic)인 것으로 나타났다. 한편, rosmarinic acid가 AlCl3에 미치는 영향에 있어서, 40 μM rosmarinic acid 처리에서는 AlCl3만의 처리에 비하여 세포생존율을 유의하게 증가시킴으로서 AlCl3의 산화적 손상으로부터 세포손상을 보호하였다. 또한 rosmarinic acid는 전자공여능을 비롯한 SOD-유사활성 및 지질과산화의 억제효과를 보임으로서 항산화능을 나타냈다. 이상의 결과로부터 산화적 손상이 AlCl3의 독성에 관여하고 있으며, rosmarinic acid는 항산화능에 의하여 AlCl3의 독성을 효과적으로 방어하였다. 따라서, rosmarinic acid와 같은 천연성분은 치매와 같이 산화적 손상과 관련된 병변의 치료적 개선을 위한 천연 소재로서의 가능성을 제시하였다.
Abstract
To examine the protective effect of rosmarinic acid on the aluminum of dementia inducer, cultured C6 glioma cells were treated with various concentrations of aluminum chloride (AlCl3) or rosmarinic acid. The cell viability, electron donating ability (EDA), superoxide dismutase (SOD)-like activity, and inhibitory activity of lipid peroxidation were evaluated for the antioxidant effect of rosmarinic acid. In these cultures, AlCl3 sowed a cytotoxic effect by decreasing the cell viability in a dose-dependent manner; then, the XTT50 value was measured at 142.2 μM of AlCl3 after treating the cultured C6 glioma cells with media containing 120∼160 μM AlCl3. Therefore, its toxicity was determined as mid-cytotoxic by Borenfreund and Puerner’s toxic criteria; while, vitamin E of antioxidant markedly increased the cell viability on AlCl3–induced cytotoxicity in these cultures. This study showed the antioxidant effect of rosmarinic acid via several assays, such as electron donating activity (EDA), superoxide dismutase (SOD)-like activity, and inhibitory activity of lipid peroxidation. From these findings, it is suggested that the oxidative stress is involved in AlCl3-induced cytotoxicity, and rosmarinic acid was effective in the protection of AlCl3-induced cytotoxicity by antioxidant activity. In conclusion, natural resources, like rosmarinic acid, may be a putative antioxidant agent for the treatment of reactive oxygen species (ROS)-mediated disease, such as dementia.
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