Neuroprotective effect of Aster yomena ethanolic extract in HT-22 and SK-N-MC cells based on antioxidant activity
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The antioxidant potentials of ethanolic extracts derived from Aster yomena (A. yomena) were evaluated by assessing their total phenolic and flavonoid contents and radical scavenging activities. Our findings revealed that the 60% ethanolic extract of A. yomena exhibited the most robust antioxidant properties among all extracts tested. Specifically, the IC50 values for the 2,2'- azino-bis (3-ethyl benzothiazoline-6-sulfonic acid) and 1,1-diphenyl-2-picrylhydrazyl radical scavenging activities of the 60% ethanolic extract from A. yomena were determined to be 1,640.30 μg/mL and 2,655.10 μg/mL, respectively. Moreover, the inhibitory effect on malondialdehyde increased with the 60% ethanolic extract from A. yomena. To assess the neuroprotective effects, we examined the impact of the 60% ethanolic extract from A. yomena against H2O2-induced cytotoxicity in HT-22 (mouse hippocampal neuronal cell line) and SK-N-MC (human neuroblastoma cell line) cells. The results demonstrated a significant improvement in cell viability and reduced intracellular oxidative stress. Furthermore, the major bioactive compounds present in the 60% ethanolic extract from A. yomena were identified as chlorogenic acid and rutin through high-performance liquid chromatography (HPLC) analysis.
Abstract
The antioxidant potentials of ethanolic extracts derived from Aster yomena (A. yomena) were evaluated by assessing their total phenolic and flavonoid contents and radical scavenging activities. Our findings revealed that the 60% ethanolic extract of A. yomena exhibited the most robust antioxidant properties among all extracts tested. Specifically, the IC50 values for the 2,2'- azino-bis (3-ethyl benzothiazoline-6-sulfonic acid) and 1,1-diphenyl-2-picrylhydrazyl radical scavenging activities of the 60% ethanolic extract from A. yomena were determined to be 1,640.30 μg/mL and 2,655.10 μg/mL, respectively. Moreover, the inhibitory effect on malondialdehyde increased with the 60% ethanolic extract from A. yomena. To assess the neuroprotective effects, we examined the impact of the 60% ethanolic extract from A. yomena against H2O2-induced cytotoxicity in HT-22 (mouse hippocampal neuronal cell line) and SK-N-MC (human neuroblastoma cell line) cells. The results demonstrated a significant improvement in cell viability and reduced intracellular oxidative stress. Furthermore, the major bioactive compounds present in the 60% ethanolic extract from A. yomena were identified as chlorogenic acid and rutin through high-performance liquid chromatography (HPLC) analysis.
참고문헌 (32)
- Abeysinghe DC, "Bioactive compounds and antioxidant capacities in different edible tissues of citrus fruit of four species", Food Chem, 104, pp.1338~1344 (2007)
- Boyles M, "Development of a standard operating procedure for the DCFH2-DA acellular assessment of reactive oxygen species produced by nanomaterials", Toxicol Mech Methods, 32, pp.439~452 (2022)
- Chang ST, "Antioxidant activity of extracts from Acacia confusa bark and heartwood", J Agric Food Chem, 49, pp.3420~3424 (2001)
- Enogieru AB, "Rutin as a potent antioxidant : Implications for neurodegenerative disorders", Oxid Med Cell Longev, 2018, pp.6241017~ (2018)
- Feng J, "Oxidative stress, the blood-brain barrier and neurodegenerative diseases : The critical beneficial role of dietary antioxidants", Acta Pharm Sin B, 13, pp.3988~4024 (2023)
- Floegel A, "Comparison of ABTS", DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods, 2011, pp.24, 1043~1048
- Gentile MT, "Neuro- nutraceutical polyphenols: How far are we?", Antioxidants, 12, pp.539~ (2023)
- Han HJ, "Industrial potential of domestic Zanthoxylum piperitum and Zanthoxylum schinifolium : Protective effect of both extracts on high glucose-induced neurotoxicity", Korean J Food Sci Technol, 52, pp.274~283 (2020)
- Heitman E, "Cognitive and neuroprotective effects of chlorogenic acid", Nutr Neurosci, 20, pp.32~39 (2017)
- Heo HJ, "Protective effect of 4', 5-dihydroxy-3', 6, 7-trimethoxyflavone from Artemisia asiatica against Aβ-induced oxidative stress in PC12 cells", Amyloid, 8, pp.194~201 (2001)
- Hwang KA, "Aster yomena extract ameliorates pro-inflammatory immune response by suppressing NF-κB activation in RAW 264. 7 cells", J Chin Med Assoc, 81, pp.102~110 (2018)
- Jeong HR, "Antioxidant activity and neuroprotective effect of ethyl acetate fraction of Lonicera japonica on H2O2-induced oxidative stress", Korean J Food Preserv, 28, pp.968~979 (2021)
- Kim GH, "The role of oxidative stress in neurodegenerative diseases", Exp Neurobiol, 24, pp.325~340 (2015)
- Kim MJ, "Determination of radical scavenging activity of Aster yomena(Kitam. )Honda", J Korea Acad Industr Coop Soc, 19, pp.402~407 (2018)
- Kim MJ, "Neuroprotective effect of Aster yomena(Kitam. )Honda against hydrogen peroxide-induced oxidative stress in SH-SY5Y cells", J Appl Biol Chem, 63, pp.283~290 (2020)
- Kowalczyk P, "Mitochondrial oxidative stress-a causative factor and therapeutic target in many diseases", Int J Mol Sci, 22, pp.13384~ (2021)
- Kuete V, "Medical Spices and Vegetables from Africa", pp.271~297 (2017), Academic Press
- Kumar G, "Neuroprotective effects of rutin against hydrogen peroxide induced oxidative stress in cerebral glioma cell line", Int J Pharm Sci Rev Res, 25, pp.304~308 (2014)
- Lee HJ, "Anti-obesity effects of Aster yomena ethanol extract in high fat diet-induced obese mice", J Physiol Pathol Korean Med, 31, pp.348~355 (2017)
- Park J, "The inhibitory effects of Aster yomena extract on microglial activation-mediated inflammatory response and pain by modulation of the NF-κB and MAPK signaling pathways", J Funct Foods, 85, pp.104659~ (2021)
- Ramalingayya GV, "Rutin protects against neuronal damage in vitro and ameliorates doxorubicin-induced memory deficits in vivo in Wistar rats", Drug Des Devel Ther, 11, pp.1011~1026 (2017)
- Ransy C, "Use of H2O2 to cause oxidative stress, the catalase issue", Int J Mol Sci, 21, pp.9149~ (2020)
- Rhew ZI, "Aster yomena has anti-arthritic activity against septic arthritis induced by Candida albicans : Its terpenoid constituent is the most effective and has synergy with indomethacin", Adv Trad Med, 20, pp.213~221 (2020)
- Rolnik A, "The plants of the Asteraceae family as agents in the protection of human health", Int J Mol Sci, 22, pp.3009~ (2021)
- Seo YM, "Antioxidative effect of Aster yomena(Kitm. )extract on C6 glioma cell line damaged by AlCl3, dementia inducer", Korean J Clin Lab Sci, 52, pp.408~416 (2020)
- Sivanantham B, "Amelioration of oxidative stress in differentiated neuronal cells by rutin regulated by a concentration switch", Biomed Pharmacother, 108, pp.15~26 (2018)
- Sun XY, "Rutin prevents tau pathology and neuroinflammation in a mouse model of Alzheimer’s disease", J Neuroinflammation, 18, pp.1~14 (2021)
- Taso OV, "Lipid peroxidation products and their role in neurodegenerative diseases", Ann Res Hosp, 3, pp.2~ (2019)
- Uddin MS, "Neuroprotective role of polyphenols against oxidative stress-mediated neurodegeneration", Eur J Pharmacol, 886, pp.173412~ (2020)
- Wang L, "The biological activity mechanism of chlorogenic acid and its applications in food industry : A review", Front Nutr, 9, pp.943911~ (2022)
- Zagoskina NV, "Polyphenols in plants : Structure, biosynthesis, abiotic stress regulation, and practical applications", Int J Mol Sci, 24, pp.13874~ (2023)
- Zhong Y, "Effects of hydrogen peroxide-induced oxidative stress on intestinal morphology, redox status, and related molecules in squabs", Animals, 13, pp.749~ (2023)