자동혈구계산기 SE-9000의 IMI Channel과 HPC mode의 유용성 평가

Abstract

SE-9000(Sysmex, Japan) automated cell counter provides an estimate of immature cells referred to as heamopoietic progenitor cells (HPC). The purpose of this study was the evaluation of the Heamatology Analyzer, SE-9000 IMI(lmmature cell information) channel, HPC mode. 140 peripheral blood stem cell collections were achieved from the stem cell collection room. The linearity and precision of HPC and IMI count and the correlation of the HPC count and CD 34+ stem cell count in peripheral blood stem cell collections. The linearities of IMI and HPC for SE-9000 was r=0.9878 and r=0.9592 each. The cefficients of vareations for IMI and HPC of SE-9000 were : for HPC(low), 114.2%; for HPC(medium), 37.9%; for HPC(lrigh), 17.9%; for IMI(low), 56.5%; for IMI (medium), 21.9%; for IMI (high) , 31.4% respedtively. IMI and HPC count showed the positive correlation with CD 34+ stem cell count with the correlation coefficients of 0.6633. The SE-9000 enables the easy and rapid determination of the IMI and HPC count. the IMI and HPC count shows good correlation with CD 34+ stema cell count by ISHAGE guideline using Flowcytometry. Whereas CD 34+ stem cell count is expensive and examined for long time. The IMI and HPC count of SE-9000 is clinically useful for monitoring the peripheral blood stem cell count.