생약추출물의 acetylcholinesterase 저해, 항산화 및 신경세포보호 효과in vitro 탐색
In vitro screening of the acetylcholinesterase inhibition, antioxidant activity, and neuronal cell protective effect of medicinal plant extracts
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초록
본 연구에서는 20종 생약 열수추출물의 AChE 활성, 산화 스트레스로 인한 지질 과산화물 생성 억제능 및 뇌신경 세포 사멸에 대한 보호효과를 비교하였다. AChE의 억제활성은 산수유, 감초, 당귀 열수추출물에서 우수하였으며, 그 중 산수유가 가장 높은 억제율을 나타내었다. 또한 H2O2/ FeSO4로 산화 스트레스를 유발시켜 생약 열수추출물의 지질과산화물 생성 억제 활성을 조사한 결과 소엽, 하수오, 계피 및 감초 열수추출물에서 높은 항산화 활성을 보였다. L-Glutamate에 의해 유도된 신경세포 독성에 대한 보호효과는 감초, 계피, 길경, 박하 열수추출물의 100 μg/mL 농도에서 관찰되었다. 본 연구를 종합적으로 살펴보았을 때, 감초 열수추출물이 치매 예방 및 개선제로써의 활용가능성이 가장 뛰어난 것으로 판단된다. 향후 치매예방 효능을 가지는 새로운 화합물 발굴을 위한 기초자료로 활용될 것으로 사료된다.
Abstract
This study investigated the effects (i.e., the acetylcholinesterase activity, lipid peroxidation, and neuronal survival) of 20 kinds of medicinal water extracts. The water extracts of three medicinal plants -- Cornus officinalis, Glycyrrhiza glabra, and Angelica gigas -- were found to be the most effective on acetylcholinesterase inhibitory activity. In the lipid peroxidation-generating system induced by H2O2/FeSO4 in rat brain homogenates, Perilla frutescens, Polygonum multiflorum, Cinnamomun cassia, and G. glabra exhibited protective activity against lipid peroxidation. The neuronal cell death induced by L-glutamate in PC12 was suppressed by the water extracts of G. glabra, Cinnamomun cassia, Platycodon grandiflorum, and Mentha arvensis at the concentration of 100 μg/mL. Taken together, these results showed that the water extract of G. glabra has the potential anti-dementia activity, which suggests that it might provide an effective strategy for improving dementia.
참고문헌 (22)
- Francis PT, "The cholinergic hypothesis of alzheimer's disease: a review of progress", J Neurol Neurosurg Psychiatry, 66, pp.137~147 (1999)
- Howes MJ R, "Plants used in Chinese and Indian traditional medicine for improvement of memory and cognitive function", Pharmacol Biochem Behav, 75, pp.513~527 (2003)
- Block F, "Flupirtine reduces functional deficits and neuronal damage after global ischemia in rats", Brain Res, 754, pp.279~284 (1997)
- Beal F, "Aging, energy and oxidative stress in neurodegenerative diseases", Ann Neurol, 38, pp.357~366 (1995)
- Coyle JT, "Alzheimer's disease: a disorder of cholinergeric innervation", Science, 219, pp.1184~1190 (1983)
- Talesa VN, "Acetylcholinesterase in Alzheimer's disease", Mech Aging Dev, 122, pp.1961~1969 (2001)
- 권영이, "청열약 추출물들의 아세틸콜린에스테라제 저해와베타아밀로이드 펩티드 응집 억제 효능", 생약학회지, 38(4), pp.308~311 (2007), 한국생약학회
- 김현주, "加味補陽還五湯과 八味合聰明湯의 microglia 보호, 항산화 및 acetylcholinesterase 억제 효과", 동의신경정신과학회지, 19(2), pp.65~76 (2008), 대한한방신경정신과학회
- Ellman GL, "A new and rapid colormetric determination of acetylcholinesterase activity", Biochem Pharmacol, 7, pp.88~95 (1961)
- Ohkawa H, "Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction", Anal Biochem, 95, pp.35~41 (1979)
- 정희록, "어성초 추출물의 항산화 및 신경세포 보호효과", 한국식품저장유통학회지, 17(5), pp.720~726 (2010), 한국식품저장유통학회
- Giacobini E, "Cholinesterase inhibitors: new roles and therapeutic alternatives", Pharmacol Res, 50, pp.433~440 (2004)
- Shao D, "Synthesis and evaluation of tacrine-E2020 hybrids as acetylcholinesterase inhibitors for the treatment of Alzheimers disease", Bioorg Med Chem Lett, 14, pp.4639~4642 (2004)
- 김정섭, "생약추출물들의 acetylcholinesterase (AChE) 저해활성검색", 생약학회지, 33(3), pp.211~218 (2002), 한국생약학회
- Park CH, "Novel cognitive improving and neuroprotective activities of Polygala tenuifolia Willdenow extract, BT-11", J Neurosci Res, 70, pp.484~492 (2002)
- Ikeya Y, "Cognitive improving and cerebral protective effects of acylated oligosaccharides in Polygala tenuifolia", Biol Pharm Bull, 27, pp.1081~1085 (2004)
- Joshi YB, "Vitamin E in aging, dementia, and Alzheimer's disease", Biofactors, 38, pp.90~97 (2012)
- Galasko DR, "Alzheimer’s disease cooperative study. Antioxidants for Alzheimer disease: a randomized clinical trial with cerebrospinal fluid biomarker measures", Arch Neurol, 69, pp.836~841 (2012)
- Chua MT, "Antioxidant activities of ethanolic extracts from the twigs of Cinnamomum osmophloeum", Bioresour Technol, 99, pp.1918~1925 (2008)
- Naito M, "Protective effects of probucol against glutamate induced cytotoxicity in neuronalcell line PC 12", Neurosci Lett, 186, pp.211~213 (1995)
- JI Yun Ahn, "Effect of Licorice (Glycyrrhiza uralensis Fisch) on Amyloid-β-induced Neurotoxicity in PC12 Cells", Food Science and Biotechnology, 19(5), pp.1391~1395 (2010), 한국식품과학회
- Shimada Y, "Extract prepared from the bark of Cinnamomum cassia Blume prevents glutamate- induced neuronal death in cultured cerebellar granule cells", Phytother Res, 14, pp.466~468 (2000)