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Bacillus lentimorbus WJ5의 감마선유도 돌연변이체들에서 공통으로 발현되는 방사선 관련 유전자의 microarray 분석

Microarray Analysis of Radiation Related Gene Expression in Mutants of Bacillus lentimorbus WJ5 Induced by Gamma Radiation

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Abstract

- To study the radiation related gene expression in mutants of Bacilluslentimorbus WJ5 induced by gamm radiation, the simultaneous gene expression wasanalyzed by DNA microarray. We constructed DNA chips including two thousandrandomly digested genome spots of B. lentimorbus WJ5 and compared its quantitativeaspect with seven mutants induced by gamma radiation (60Co). From the clusteranalysis of gene expression pattern, totally 408 genes were expressed and 27 genesgenes involved in repair( mutL, mutM ), energy metabolism( acsA, sdhB, pgk, yhjB , citB),protease ( npr ), and reduction response to oxidative stress (HMM) were simultaneouslyupregulated. It seems that the induction of the direct and/or indirect repair relatedgenes in mutants induced by gamma radiation could be remarkably different from theadaptive responses against acute exposure to radiation.

참고문헌 (22)

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