Induction of SOS Genes by a Low Dose of Gamma Radiation, 10 Gy, in Salmonella enterica Serovar Typhimurium
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
In a previous study, a relatively high dose of gamma radiation (1 kGy) did not fullyinduce typical SOS genes such as sulA, recA, recN, and din in Salmonella Typhimurium (S. Typhimurium)(Lim et al. 2008, Gene expression profiles following high-dose exposure to gamma radiationin Salmonella enterica serovar Typhimuium. J. Radiat. Ind. 3:111-119). In this study, we examinedchanges in the transcriptional repertoire of S. Typhimurium after a dose of 10 Gy using DNAmicroarrays. It was found that more than half (~65%) of the 26 up-regulated genes belong to theSOS regulon: ten genes are typical SOS genes, and seven genes are Salmonella prophage genes,which are known to be activated by LexA cleavage. Among 29 down-regulated genes, the functionof five genes with the most decreased expression is associated with carbohydrate transport andenergy production. This suggests that upon exposure to gamma radiation cells may cease growingby reducing the metabolic activity, and repair DNA damage using a DNA repair system such asthe SOS response system. The difference in expression of the SOS genes between a high (1 kGy)and low (10 Gy) dose of radiation shows the possibility that cells may opt for one of multiple regulatorycircuits in response to the specific gamma radiation dose.
Abstract
In a previous study, a relatively high dose of gamma radiation (1 kGy) did not fullyinduce typical SOS genes such as sulA, recA, recN, and din in Salmonella Typhimurium (S. Typhimurium)(Lim et al. 2008, Gene expression profiles following high-dose exposure to gamma radiationin Salmonella enterica serovar Typhimuium. J. Radiat. Ind. 3:111-119). In this study, we examinedchanges in the transcriptional repertoire of S. Typhimurium after a dose of 10 Gy using DNAmicroarrays. It was found that more than half (~65%) of the 26 up-regulated genes belong to theSOS regulon: ten genes are typical SOS genes, and seven genes are Salmonella prophage genes,which are known to be activated by LexA cleavage. Among 29 down-regulated genes, the functionof five genes with the most decreased expression is associated with carbohydrate transport andenergy production. This suggests that upon exposure to gamma radiation cells may cease growingby reducing the metabolic activity, and repair DNA damage using a DNA repair system such asthe SOS response system. The difference in expression of the SOS genes between a high (1 kGy)and low (10 Gy) dose of radiation shows the possibility that cells may opt for one of multiple regulatorycircuits in response to the specific gamma radiation dose.
참고문헌 (16)
- Bossi L, "Phages:their role in bacterial pathogenesis and biotechnology", pp.165~186 (2005), ASM Press
- Bunny K, "Phenotypes of lexA mutations in Salmonella enterica : evidence for a lethal lexA null phenotype due to the Fels-2 prophage", J. Bacteriol, 184, pp.6235~6249 (2002)
- Chinag SM, "Regulators of oxidative stress response genes in Escherichia coli and their functional conservation in bacteria", Arch. Biochem. Biophys, 525, pp.161~169 (2012)
- Farr SB, "Oxidative stress responses in Escherichia coli and Salmonella typhimurium", Microbiol. Rev, 55, pp.561~585 (1991)
- Fernandez de Henestrosa AR, "Identification of additional genes belonging to the LexA regulon in Escherichia coli", Mol. Microbiol, 35, pp.1560~1572 (2000)
- Imlay JA, "Pathways of oxidative damage", Annu. Rev. Microbiol, 57, pp.395~418 (2003)
- Janion C, "Some aspects of the SOS response system-A critical survey", Acta Biochim. Pol, 48, pp.599~610 (2001)
- Lemire S, "Bacteriophage crosstalk : coordination of prophage induction by trans-acting antirepressors", PLoS Genet, 7, pp.e1002149~ (2011)
- Lim S, "Gene expression profiles following high-dose exposure to gamma radiation in Salmonella enterica serovar Typhimuium", J. Radiat. Ind, 3, pp.111~119 (2008)
- 임상용, "Proteome Analysis of Escherichia coli after High-dose Radiation", 방사선산업학회지, 5(1), pp.1~5 (2011), (사)한국방사선산업학회
- McClelland M, "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2", Nature, 413, pp.852~856 (2001)
- Michel B, "After 30 years of study, the bacterial SOS response still surprises us", PLoS Biol, 3, pp.e255~ (2005)
- Riley PA, "Free radicals in biology : oxidative stress and the effects of ionizing radiation", Int. J. Radiat. Biol, 65, pp.27~33 (1994)
- Saier MH Jr, "Multiple mechanisms controlling carbon metabolism in bacteria", Biotechnol. Bioeng, 58, pp.170~174 (1998)
- Storz G, "Oxidative stress", Curr. Opin. Microbiol, 2, pp.188~194 (1999)
- Waldor MK, "Phage regulatory circuits and virulence gene expression", Curr. Opin. Microbiol, 8, pp.459~465 (2005)