PCR (polymerase chain reaction)법과 조직학적 방법을 통한 넙치 조직에서의 Kudoa septempunctata (Myxosporea: Multivalvulida)의 분석에 관한 연구
A Study About Analysis Results for Kudoa septempunctata (Myxosporea: Multivalvulida) in Tissue at Olive Flounder, using PCR (polymerase chain reaction) and Histological Methods
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초록
Kudoa septempunctata에 대한 PCR 검사에서 양성으로 확 인된 개체를 조직 내 포자가 확인된 개체 (3마리)와 포자가 확인되지 않은 개체 (4마리)를 선별하여 순치 후 혈액과 조 직검사를 실시하였다. 혈액에 대하여 PCR 검사를 실시하여 포자가 명확하게 확인되었던 2개체에서는 음성이 확인되었 으며, 다른 5개체는 감도는 각각 차이는 있었지만 양성으로 확인되었다. 포자가 확인된 개체의 각 장기 (간, 비장, 신장, 심장, 위, 소화관, 근육)에 대하여 PCR 검사를 실시한 결과, 심장과 근육에서 명확한 양성이 나타났다. 근육조직의 H&E 염색과 ISH를 실시하여 K. septempunctata의 감염으로 근섬 유에 괴사가 발생하고, 이 부위에 포자의 슈도시스트가 형성 되는 것을 확인하였으나 심한 염증반응을 유도하지는 않는 것이 확인되었다. 이후 혈액에 대한 더욱 면밀한 조사가 필 요할 것으로 보이며, 연구내용은 넙치양식에서의 쿠도아감 염증의 예방과 구제를 위한 기반자료가 될 것으로 기대된다.
Abstract
This study is for the consideration of the existence tendency of Kudoa septempunctata in olive flounder. In general, muscle has shown a strong PCR positive reaction in spores containing tissues rather than non-containing tissues. However, blood PCR results showed opposed tendency. In various organs of the tested fish containing spores in muscle tissue, heart had shown positive reaction along with muscle at PCR analysis. Muscle fiber necrosis was observed at the histological observation, and this degeneration was common in both samples. The one sample was the PCR positive muscle containing spore and the other was the PCR positive muscle non-containing spore. Both of muscle tissues indicated a positive reaction at ISH (in-situ hybridization) against K. septempunctata.
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