토양에 매몰 방제된 화상병 감염 사과와 배 나무로부터 화상병균 생존 조사
Investigating Survival of Erwinia amylovora from Fire Blight-Diseased Apple and Pear Trees Buried in Soil as Control Measure
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참고문헌
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초록
BACKGROUND: Since 2015, fire blight disease caused by Erwinia amylovora has been devastating apple and pear orchards every year. To quickly block the disease spreading, infected apple and pear trees have been buried in soil. However, concern on the possibility of the pathogen survival urgently requires informative data on the buried host plants. Therefore, this study was conducted to investigate the survival of the pathogen from the buried host plants. METHODS AND RESULTS: Apple trees buried in 42 months ago in a Jecheon site and pear trees buried in 30 months ago in an Anseong site were excavated using an excavator. Plant samples were taken from stems and twigs of the excavated trees. The collected 120 samples were checked for rotting and used for bacterial isolation, using TSA, R2A, and E. amylovora selection media. The purely isolated bacteria were identified based on colony morphology and 16S rDNA sequences. Wood rotting and decay with off smells and discoloring were observed from the samples. A total of 17 genera and 48 species of bacteria were identified but E. amylovora was not detected. CONCLUSION: Our investigation suggests that the survival of E. amylovora doesn’t seem possible in the infected hosts which have been buried in soil for at least 30 months. Therefore, the burial control can be considered as a safe method for fire blight disease.
Abstract
BACKGROUND: Since 2015, fire blight disease caused by Erwinia amylovora has been devastating apple and pear orchards every year. To quickly block the disease spreading, infected apple and pear trees have been buried in soil. However, concern on the possibility of the pathogen survival urgently requires informative data on the buried host plants. Therefore, this study was conducted to investigate the survival of the pathogen from the buried host plants. METHODS AND RESULTS: Apple trees buried in 42 months ago in a Jecheon site and pear trees buried in 30 months ago in an Anseong site were excavated using an excavator. Plant samples were taken from stems and twigs of the excavated trees. The collected 120 samples were checked for rotting and used for bacterial isolation, using TSA, R2A, and E. amylovora selection media. The purely isolated bacteria were identified based on colony morphology and 16S rDNA sequences. Wood rotting and decay with off smells and discoloring were observed from the samples. A total of 17 genera and 48 species of bacteria were identified but E. amylovora was not detected. CONCLUSION: Our investigation suggests that the survival of E. amylovora doesn’t seem possible in the infected hosts which have been buried in soil for at least 30 months. Therefore, the burial control can be considered as a safe method for fire blight disease.
참고문헌 (10)
- Cohen, S. N., "Nonchromosomal antibiotic resistance in bacteria:genetic transformation of Escherichia coli by R-factor DNA", Proceedings of the National Academy of Sciences, 69(8), pp.2110~2114 (1972)
- Crosse, J. E., "A selective medium for and a definitive colony characteristic of Erwinia amylovora", Phytopathology, 63(11), pp.1425~1426 (1973)
- Drenova, N. V., "Bacterial fire blight in the Republic of Kazakhstan", Plant Health Research and Practice, 1, pp.44~48 (2012)
- Kim, W. S., "Molecular comparison of pathogenic bacteria from pear trees in Japan and the fire blight pathogen Erwinia amylovora", Microbiology, 147(Pt 11), pp.2951~2959 (2001)
- Myung, I. S., "First report of fire blight caused by Erwinia amylovora on Chinese quince in South Korea", Plant Disease, 100(12), pp.2521~ (2016)
- Park, D. H., "First report of fire blight disease on Asian pear caused by Erwinia amylovora in Korea", Plant Disease, 100(9), pp.1946~ (1946)
- Gagelidzea, N. L., "Bacterial composition of different types of soils of Georgia", Annals of Agrarian Science, 16, pp.17~21 (2018)
- Gusberti, M., "Fire blight control : the struggle goes on. A comparison of different fire blight control methods in Switzerland with respect to biosafety, efficacy and durability", International Journal of Environmental Research and Public Health, 12(9), pp.11422~11447 (2015)
- Yoon, S. H., "Introducing EzBioCloud : A taxonomically united database of 16S rRNA and whole genome assemblies", International Journal of Systematic and Evolutionary Microbiology, 67(5), pp.1613~1617 (2017)
- Zwet, T., "Fire blight, a bacterial disease of Rosaceous plants", pp.2~18 (1979), USDA