엽록소 형광 기술을 활용한 배나무 화상병 방제 약제 효과 분석
Effectiveness of bactericide for controlling fireblight of pear tree using chlorophyll fluorescence technology
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초록
화상병의 빠른 확산으로 농촌진흥청은 약제를 농가에 보급하고 있으나, 주성분 약제에 대한 화상병 억제 효과에 대한 연구는 부족한 상황이다. 엽록소 형광 분석은 식물의 광합성 효율을 비파괴적으로 분석하여 병원균 진전에 따른 시계열 데이터 분석이 가능하고 대량 분석할 수 있다는 장점이 있다. 본 연구는 엽록소 형광 분석을 통해 주성분 약제에 대한 화상병 억제 효과를 분석하였다. 개화 상태의 배나무(신고 3년생)를 대상으로 방제 약제와 화상병원균을 꽃에 분무 처리 및 접종하고, 7일 간격으로 엽록소 형광 반응을 측정했다. 약제 처리 28일 후 약제 무처리구는 약제 처리구 평균 대비 O-J 전이단계의 형광량이 2배 높게 나타났는데, 이는 병원균 감염으로 잎이 고사하여 광합성계 II (PSII) 공여체의 전자전달 저해가 발생했기 때문인 것으로 추정된다. 또한, 전자전달효율(ET2o, RE1o)이 감소하고 광화학적 구동력(DF total ABS)이 약제 처리구 평균 대비 20% 수준으로 감소한 것으로 나타났는데, 이는 엽록소의 손상과 광합성 능력의 감소를 의미한다. 결론적으로, 엽록소 형광 기술을 활용하여 배나무 개화기 화상병 방제 효과를 정량적으로 평가할 수 있을 것으로 사료된다.
Abstract
Due to rapid spread of fireblight, the Rural Development Administration is supplying bactericides to farmers. However, research on inhibitory effects of main active ingredients in these bactericides on fireblight is lacking. Chlorophyll fluorescence analysis is a non-destructive method for analyzing the photosynthetic efficiency of plants, enabling time-series data analysis of pathogen progression and allowing for large-scale studies. Therefore, this study analyzed inhibitory effects of main active ingredients in bactericides on fireblight using chlorophyll fluorescence response analysis. Flowering pear trees (three-year-old ‘Shingo’ variety) were sprayed with control agents and fire blight pathogens on flowers. Chlorophyll fluorescence responses were then measured at seven-day intervals. Twenty-eight days after bactericide treatment, the fluorescence of the O-J transition stage in the untreated group was twice as high as in the average bactericide-treated group presumably due to inhibition of electron transport in the PSII donor side caused by pathogen infection, leading to leaf necrosis. Additionally, the electron transport efficiency (ET2o, RE1o) decreased, reducing the driving force of photosynthesis (DF total ABS) to 20% of the average bactericide[1]treated group, indicating chlorophyll damage and reduced photosynthetic capacity. In conclusion, chlorophyll fluorescence technology can be used to quantitatively evaluate the efficacy of fire blight control agents during the flowering period of pear trees.
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