전라북도 지역 농업용수의 미생물학적 특성 및 온도와 수질에 따른 농업용수의 병원성대장균 O157:H7 밀도 변화
Microbiological Quality of Agricultural Water in Jeollabuk-do and the Population Changes of Pathogenic Escherichia Coli O157:H7 in Agricultural Water Depending on Temperature and Water Quality
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
BACKGROUND: Agricultural water is known to be one of the major routes in bacterial contamination of fresh vegetable. However, there is a lack of fundamental data on the microbial safety of agricultural water in Korea. METHODS AND RESULTS: We investigated the density of indicator bacteria in the surface water samples from 31 sites collected in April, July, and October 2018, while the groundwater samples were collected from 20 sites within Jeollabuk-do in April and July 2018. In surface water, the mean density of coliform, fecal coliform, and Escherichia coli was 2.7±0.55, 1.9±0.71, and 1.4±0.58 log CFU/100 mL, respectively, showing the highest bacterial density in July. For groundwater, the mean density of coliform, fecal coliform, and E. coli was 1.9±0.58, 1.4±0.37, and 1.0±0.33 log CFU/ 100mL, respectively, showing no significant difference between sampling time. The survival of E. coli O157:H7 were prolonged in water with higher organic matter contents such as total nitrogen (TN), and nitrate-nitrogen (NO3-N). The reduction rates of E. coli O157:H7 in the water showed greater in order of 25, 35, 5, and 15℃. CONCLUSION: These results can be utilized as fundamental data for prediction the microbiological contamination of agricultural water and the development of microbial prevention technology.
Abstract
BACKGROUND: Agricultural water is known to be one of the major routes in bacterial contamination of fresh vegetable. However, there is a lack of fundamental data on the microbial safety of agricultural water in Korea. METHODS AND RESULTS: We investigated the density of indicator bacteria in the surface water samples from 31 sites collected in April, July, and October 2018, while the groundwater samples were collected from 20 sites within Jeollabuk-do in April and July 2018. In surface water, the mean density of coliform, fecal coliform, and Escherichia coli was 2.7±0.55, 1.9±0.71, and 1.4±0.58 log CFU/100 mL, respectively, showing the highest bacterial density in July. For groundwater, the mean density of coliform, fecal coliform, and E. coli was 1.9±0.58, 1.4±0.37, and 1.0±0.33 log CFU/ 100mL, respectively, showing no significant difference between sampling time. The survival of E. coli O157:H7 were prolonged in water with higher organic matter contents such as total nitrogen (TN), and nitrate-nitrogen (NO3-N). The reduction rates of E. coli O157:H7 in the water showed greater in order of 25, 35, 5, and 15℃. CONCLUSION: These results can be utilized as fundamental data for prediction the microbiological contamination of agricultural water and the development of microbial prevention technology.
참고문헌 (19)
- Allende, A., "Irrigation water quality for leafy crops : a perspective of risks and potential solutions", International Journal of Environmental Research and Public Health, 12(7), pp.7457~7477 (2015)
- Auld, H., "Heavy rainfall and waterborne disease outbreaks: the Walkerton example", Journal of Toxicology and Environmental Health, Part A, 67(20-22), pp.1879~1887 (2004)
- Clavero, M., "Survival of Escherichia coli O157 : H7 in broth and processed salami as influenced by pH, water activity, and temperature and suitability of media for its recovery", Applied and Environmental Microbiology, 62(8), pp.2735~2740 (1996)
- Czajkowska, D., "Survival of Escherichia coli serotype O157 : H7 in water and in bottom-shore sediments", Polish Journal of Environmental Studies, 14(4), pp.423~430 (2005)
- Divya, A., "Effects of some water quality parameters especially total coliform and fecal coliform in surface water of Chalakudy river", Procedia Technology, 24, pp.631~638 (2016)
- Henis, Y., "Factors involved in multiplication and survival of Escherichia coli in lake water", Microbial ecology, 17(2), pp.171~180 (1989)
- Kleinheinz, G. T., "Effects of rainfall on E. coli concentrations at Door County, Wisconsin beaches", International Journal of Microbiology, 2009, pp.1~9 (2010)[DOI]
- Korhonen, L. K., "Survival of Escherichia coli and Campylobacter jejuni in untreated and filtered lake water", Journal of Applied Bacteriology, 71(4), pp.379~382 (1991)
- LeChevallier, M. W., "Full-scale studies of factors related to coliform regrowth in drinking water", Applied and Environmental Microbiology, 62(7), pp.2201~2211 (1996)
- Mikhail, A. F. W., "An outbreak of Shiga toxinproducing Escherichia coli O157 : H7 associated with contaminated salad leaves : epidemiological, genomic and food trace back investigations", Epidemiology &Infection, 146(2), pp.187~196 (2018)
- 오소영, "유기농 시설엽채류의 유해미생물 오염평가", 한국식품위생안전성학회지, 33(1), pp.31~37 (2018), 한국식품위생안전성학회[DOI]
- Pachepsky, Y., "Can E. coli or thermotolerant coliform concentrations predict pathogen presence or prevalence in irrigation waters?", Critical Reviews in Microbiology, 42(3), pp.384~393 (2016)
- Pagadala, S., "Assessment of region, farming system, irrigation source and sampling time as food safety risk factors for tomatoes", International Journal of Food Microbiology, 196, pp.98~108 (2015)
- Steele, M., "Irrigation water as source of foodborne pathogens on fruit and vegetables", Journal of food protection, 67(12), pp.2839~2849 (2004)
- Tran, N. H., "Fecal pollution source tracking toolbox for identification, evaluation and characterization of fecal contamination in receiving urban surface waters and groundwater", Science of the Total Environment, 538, pp.38~57 (2015)
- Uyttendaele, M., "Microbial hazards in irrigation water : Standards, norms, and testing to manage use of water in fresh produce primary production", Comprehensive Reviews in Food Science and Food Safety, 14(4), pp.336~356 (2015)
- Van Der Linden, I., "Enteric pathogen survival varies substantially in irrigation water from belgian lettuce producers", International Journal of Environmental Research and Public Health, 11(10), pp.10105~10124 (2014)
- Wang, G., "Survival of enterohemorrhagic Escherichia coli O157 : H7 in water", Journal of food protection, 61(6), pp.662~667 (1998)
- Zhang, J., "Effects of physico-chemical parameters on the bacterial and fungal communities during agricultural waste composting", Bioresource Technology, 102(3), pp.2950~2956 (2011)