병저항성 GM(OsCK1)벼가 미꾸리(Misgurnus anguillicaudatus)및 잉어(Cyprinus carpio)에 미치는 영향
Responses of Misgurnus anguillicaudatus and Cyprinus carpio Fed on Disease Resistant(OsCK1) Rice Variety
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BACKGROUND: The disease resistant (OsCK1) rice was generated by inserting choline kinase (CK1) and phosphinothricin acetyltransferase (PAT) genes isolated from Oriza sativa and Streptomyces hygroscopicus into the genome of rice (Nakdongbyeo). With the potential problems of safety, the non-target organism evaluation is required as an essential element for the environmental risk assessment of genetically modified (GM) crops. In present study, we studied the effects on survival of Misgurnus anguillicaudatus and Cyprinus carpio, commonly used as a model organism in ecotoxicological studies. METHODS AND RESULTS: The M. anguillicaudatus and C. carpio were fed on disease resistant (OsCK1) rice and non-genetically modified (non-GM) rice (Nakdongbyeo) to 0, 10, 100, 1,000 and 5,000 mg/L, as treatment concentration respectively. The OsCK1 rice used for the test was confirmed to have the OsCK1/PAT gene expression by the PCR and ELISA analysis. Feeding test showed that no significant differences in cumulative immobility and abnormal response of M. anguillicaudatus and C. carpio fed on between OsCK1 rice and non-GM rice. The 96hr-LC50 values showed no difference between OsCK1 rice (>5,000 mg/L) and non-GM rice (>5,000mg/L). CONCLUSION(S): The results of this study suggested that there was no significant difference in toxicity for M. anguillicaudatus and C. carpio between OsCK1 rice and non-GM counterparts.
Abstract
BACKGROUND: The disease resistant (OsCK1) rice was generated by inserting choline kinase (CK1) and phosphinothricin acetyltransferase (PAT) genes isolated from Oriza sativa and Streptomyces hygroscopicus into the genome of rice (Nakdongbyeo). With the potential problems of safety, the non-target organism evaluation is required as an essential element for the environmental risk assessment of genetically modified (GM) crops. In present study, we studied the effects on survival of Misgurnus anguillicaudatus and Cyprinus carpio, commonly used as a model organism in ecotoxicological studies. METHODS AND RESULTS: The M. anguillicaudatus and C. carpio were fed on disease resistant (OsCK1) rice and non-genetically modified (non-GM) rice (Nakdongbyeo) to 0, 10, 100, 1,000 and 5,000 mg/L, as treatment concentration respectively. The OsCK1 rice used for the test was confirmed to have the OsCK1/PAT gene expression by the PCR and ELISA analysis. Feeding test showed that no significant differences in cumulative immobility and abnormal response of M. anguillicaudatus and C. carpio fed on between OsCK1 rice and non-GM rice. The 96hr-LC50 values showed no difference between OsCK1 rice (>5,000 mg/L) and non-GM rice (>5,000mg/L). CONCLUSION(S): The results of this study suggested that there was no significant difference in toxicity for M. anguillicaudatus and C. carpio between OsCK1 rice and non-GM counterparts.
참고문헌 (20)
- De Vries J., "Microbial horizontal gene transfer and the DNA release from transgenic crop plants", Plant and Soil, 266, pp.91~104 (2005)
- Ferguson, H. W., "Gill and pseudobranchs In: Sysremic pathology of fish", pp.11~40 (1989), Iowa State Univercity
- Ha, S. H., "Application of Two Bicistronic Systems Involving 2A and IRES Sequences to the Biosynthesis of Carotenoids in Rice Endosperm", Plant Biotechnology Journal, 8, pp.928~938 (2010)
- The global status of commercialized biotech, "GM crops: 2012", James, C., 2012, ISAAA Briefs 44
- Jung, S., "Dual targeting of Myxococcus xanthus protoporphyrinogen oxidase into chloroplasts and mitochondria and high level oxyfluorfen resistance", Plant Cell Environ, 27, pp.1436~1446 (2004)
- 김자현, "용담댐 건설후 하류부 하천 생태계의 탁수영향 평가", 생태와 환경, 40(1), pp.130~142 (2007), 한국하천호수학회
- 김금용, "물벼룩을 이용한 중금속 급성 독성 실험", 한국수처리학회지, 18(3), pp.37~47 (2010), 한국수처리학회
- Kim, H. J., "Expression of PAT and NPT II proteins during the developmental stages of a genetically modified pepper developed in Korea", Food Sci. Biotechnol, 22, pp.195~200 (2010)
- Kim, H. J., "Quantification of mCry1Ac1 and PAT Proteins over time in genetically modified pepper developed in Korea", Food Chem, 58 (2013)
- 이현숙, "대규모 GMO 포장에서 비타민 A 강화 벼의 농업특성 검정", 한국육종학회지, 42(1), pp.56~60 (2010), 한국육종학회
- Lee, J. S., "OsCK1 gene from Oryza sativa, expression vector comprising the gene, transformants transformed with the vector and production method of the transformants", Korean Patent No. 100682129, 100682129~ (2007)
- 오성덕, "해충저항성 Bacillus thuringiensis (Bt) 벼의 환경위해성 평가: 해충저항성 Bt벼가 물벼룩(Daphnia magna)에 미치는 영향", Journal of Applied Biological Chemistry, 54(4), pp.296~302 (2011), 한국응용생명화학회
- 오성덕, "해충저항성 Bt벼의 환경위해성 평가: 해충저항성 Bt벼가 미꾸리(Misgurnus anguillicaudatus) 및 잉어(Cyprinus carpio)에 미치는 영향", 한국국제농업개발학회지, 23(5), pp.570~577 (2011), 한국국제농업개발학회
- 오성덕, "비타민A 강화 벼 분자생물학적 분석 및 물벼룩 급이 효과", 한국국제농업개발학회지, 24(4), pp.477~484 (2012), 한국국제농업개발학회
- Oh, S. K., "CaMsrB2, pepper methionine sulfoxide reductase B2, is a novel defense regulator against oxidative stress and pathogen attack", Plant Physiol, 154, pp.245~261 (2010)
- Shin, K. S., "Research articles : Molecular biological characteristics and analysis using the specific markers of leaf folder-resistant GM rice", Plant Biotechnology, 36, pp.115~123 (2009)
- Shin, K. S., "Detection of high α-tocopherol GM perilla developed in Korea", J Intl Agri, 22, pp.399~405 (2010)
- 신명자, "잉어(Cyprinus carpio)에서 탁수 노출에 의한 아가미 미세구조 변화와 유전자 확인", 생태와 환경, 44(1), pp.95~101 (2011), 한국하천호수학회
- Toriyama, K., "Transgenic rice plants agter direct gene transfer into protoplasts", Nat Biotechnol, 6, pp.1072~1074 (1988)
- Versteeg, D. J., "Ceriodaphnia and Daphnia: A comparison of their sensitivity to xenobiotics and utility as a test species", Chemosphere, 34, pp.869~892 (1997)