병저항성 GM(OsCK1)벼의 물벼룩(Daphnia magna)에 대한급성독성 평가
Acute Toxicity Evaluation to Daphnia magna of Disease Resistant(OsCK1) Rice
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초록
BACKGROUND: The disease resistant (OsCK1) rice wasgenerated by inserting choline kinase (CK1) andphosphinothricin acetyltransferase (PAT) genes isolatedfrom Oryza sativa and Streptomyces hygroscopicus into thegenome of the rice, Nakdongbyeo. With the potentialproblems of safeties, the evaluations on non-targetorganisms are essentially required for the environmentalrisk assessment of genetically modified (GM) crops. In thepresent study, we conducted the evaluation of acute toxicityon Daphnia magna that commonly used as a modelorganism in ecotoxicological studies for non-targetorganism evaluation. METHODS AND RESULTS: Effect of acute toxicity toDaphnia magna by each concentration were investigated inthe disease resistant (OsCK1) rice and non-geneticallymodified (non-GM) rice, Nakdongbyeo, as concentration(0, 1,000, 1,800, 3,240, 5,830, 10,500 and 20,000 mg/L). The OsCK1 rice used for the test was confirmed to expressthe OsCK1/PAT gene by the PCR(Polymerase chainreaction) and western blot analysis. Feeding test showedthat no significant differences in cumulative immobilityand abnormal response of Daphnia magna fed on OsCK1rice or non-GM rice. The 48hr-EC50 values showed nodifference between OsCK1 rice (3,147.18 mg/L) andnon-GM rice (3,596.27 mg/L). CONCLUSION: This result suggested that there was nosignificant difference in toxicity to Daphnia magnabetween OsCK1 rice and non-GM counterpart.
Abstract
BACKGROUND: The disease resistant (OsCK1) rice wasgenerated by inserting choline kinase (CK1) andphosphinothricin acetyltransferase (PAT) genes isolatedfrom Oryza sativa and Streptomyces hygroscopicus into thegenome of the rice, Nakdongbyeo. With the potentialproblems of safeties, the evaluations on non-targetorganisms are essentially required for the environmentalrisk assessment of genetically modified (GM) crops. In thepresent study, we conducted the evaluation of acute toxicityon Daphnia magna that commonly used as a modelorganism in ecotoxicological studies for non-targetorganism evaluation. METHODS AND RESULTS: Effect of acute toxicity toDaphnia magna by each concentration were investigated inthe disease resistant (OsCK1) rice and non-geneticallymodified (non-GM) rice, Nakdongbyeo, as concentration(0, 1,000, 1,800, 3,240, 5,830, 10,500 and 20,000 mg/L). The OsCK1 rice used for the test was confirmed to expressthe OsCK1/PAT gene by the PCR(Polymerase chainreaction) and western blot analysis. Feeding test showedthat no significant differences in cumulative immobilityand abnormal response of Daphnia magna fed on OsCK1rice or non-GM rice. The 48hr-EC50 values showed nodifference between OsCK1 rice (3,147.18 mg/L) andnon-GM rice (3,596.27 mg/L). CONCLUSION: This result suggested that there was nosignificant difference in toxicity to Daphnia magnabetween OsCK1 rice and non-GM counterpart.
참고문헌 (20)
- Brookes, G., "Global impact of biotech crops: Socio-economic and environmental effects in the first ten years of commercial use", AgBioForum, 9, pp.139~151 (2006)
- De Vries, J., "Microbial horizontal gene transfer and the DNA release from transgenic crop plants", Plant and Soil, 266, pp.91~104 (2005)
- Ha, S.H., "Application of two bicistronic systems involving 2A and IRES sequences to the biosynthesis of carotenoids in rice endosperm", Plant Biotechnol. J, 8, pp.928~938 (2010)
- The global status of commercialized biotech, "GM crops: 2013", James, C., 2013, ISAAA Brief No. 46
- Jung, S., "Dual targeting of Myxococcus xanthus protoporphyrinogen oxidase into chloroplasts and mitochondria and high level oxyfluorfen resistance", Plant Cell Environ, 27, pp.1436~1446 (2004)
- 김금용, "물벼룩을 이용한 중금속 급성 독성 실험", 한국수처리학회지, 18(3), pp.37~47 (2010), 한국수처리학회
- Kim, H.J., "Expression of PAT and NPT II proteins during the developmental stages of a genetically modified pepper developed in Korea", Food Sci. Biotechnol, 22, pp.195~200 (2010)
- Kim, H.J., "Quantification of mCry1Ac1 and PAT Proteins over time in genetically modified pepper developed in Korea", Food Chem, 58, pp.10906~10910 (2013)
- Lee, J.S., "OsCK1 gene from Oryza sativa, expression vector comprising the gene, transformants transformed with the vector and production method of the transformants", Korean Patent No. 100682129, ~ (2007)
- 오성덕, "해충저항성 Bacillus thuringiensis (Bt) 벼의 환경위해성 평가: 해충저항성 Bt벼가 물벼룩(Daphnia magna)에 미치는 영향", Journal of Applied Biological Chemistry, 54(4), pp.296~302 (2011), 한국응용생명화학회
- 오성덕, "해충저항성 Bt벼의 환경위해성 평가: 해충저항성 Bt벼가 미꾸리(Misgurnus anguillicaudatus) 및 잉어(Cyprinus carpio)에 미치는 영향", 한국국제농업개발학회지, 23(5), pp.570~577 (2011), 한국국제농업개발학회
- 오성덕, "해충저항성 Bacillus thuringiensis (Bt) 벼 급이가 벼물바구미(Lissorhoptrus oryzophilus)의 살충제 감수성에 미치는 영향", 한국국제농업개발학회지, 24(2), pp.247~253 (2012), 한국국제농업개발학회[DOI]
- 오성덕, "비타민 A 강화벼 급이가 벼물바구미(Lissorhoptrus oryzophilus)의 살충제 감수성에 미치는 영향", 한국환경농학회지, 31(3), pp.286~292 (2012), 한국환경농학회[DOI]
- 오성덕, "비타민A 강화 벼 분자생물학적 분석 및 물벼룩 급이 효과", 한국국제농업개발학회지, 24(4), pp.477~484 (2012), 한국국제농업개발학회
- 오성덕, "병저항성 GM(OsCK1)벼가 미꾸리(Misgurnus anguillicaudatus)및 잉어(Cyprinus carpio)에 미치는 영향", 한국환경농학회지, 32(3), pp.231~239 (2013), 한국환경농학회[DOI]
- Oh, S.K., "CaMsrB2, pepper methionine sulfoxide reductase B2, is a novel defense regulator against oxidative stress and pathogen attack", Plant Physiol, 154, pp.245~261 (2010)
- Owen, M.D.K., "Current use of transgenic herbicide resistant soybean and corn in the USA", Crop Prot, 19, pp.765~771 (2000)
- 신공식, "혹명나방저항성 GM 벼의 분자생물학적 특성 및 특이 마커를 이용한 검정", Journal of Plant Biotechnology, 36(2), pp.115~123 (2009), 한국식물생명공학회
- Thomas, B., "Demographic responses of Daphnia magna fed transgenic Bt-maize", Ecotoxicology, 19, pp.419~430 (2010)
- Toriyama, K., "Transgenic rice plants after direct gene transfer into protoplasts", Nat. Biotechnol, 6, pp.1072~1074 (1988)