병저항성(OsCK1) 유전자변형 벼 유전자 이동성 평가
Assessment of Gene Flow in Disease Resistant (OsCK1) Genetically Modified Rice
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참고문헌
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초록
Although genetically modified (GM) crops have never been cultivated commercially in Korea, it is necessary for athorough assessment of the risks associated with their environmental release. We determined the frequency of pollen mediatedgene flow from disease resistant GM rice (OsCK1) to non-GM rice (Nagdongbyeo) and weedy rice (R55). A total of 449,711or 164,604 seeds were collected from non-GM and weedy rice, respectively which were planted around OsCK1. Resistance ofthe hybrids was determined by repeated spraying of herbicide and DNA analysis using specific primer to confirm hybrids. Thoughnon-GM rice and weedy rice have similar flowering time, the hybrids were found only in non-GM rice and out-crossing rangedfrom 0.018% at 0.3 m to 0.013% at 0.6 m. All of hybrids were located within 0.6 m distance from the GM rice plot in southerlydirection. The meteorological factors including temperature and relative humidity during flowering time were found to be themost important factors for determining rice out-crossing. It should be considered many factors like the local weather conditionand flowering time to set up the safety management policy to prevent pollen mediated gene flow between GM and conventionalcrop.
Abstract
Although genetically modified (GM) crops have never been cultivated commercially in Korea, it is necessary for athorough assessment of the risks associated with their environmental release. We determined the frequency of pollen mediatedgene flow from disease resistant GM rice (OsCK1) to non-GM rice (Nagdongbyeo) and weedy rice (R55). A total of 449,711or 164,604 seeds were collected from non-GM and weedy rice, respectively which were planted around OsCK1. Resistance ofthe hybrids was determined by repeated spraying of herbicide and DNA analysis using specific primer to confirm hybrids. Thoughnon-GM rice and weedy rice have similar flowering time, the hybrids were found only in non-GM rice and out-crossing rangedfrom 0.018% at 0.3 m to 0.013% at 0.6 m. All of hybrids were located within 0.6 m distance from the GM rice plot in southerlydirection. The meteorological factors including temperature and relative humidity during flowering time were found to be themost important factors for determining rice out-crossing. It should be considered many factors like the local weather conditionand flowering time to set up the safety management policy to prevent pollen mediated gene flow between GM and conventionalcrop.
참고문헌 (16)
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