한약재 추출물의 항산화작용 및 아질산염 소거작용
Antioxidative and Nitrite Scavenging Abilities of Medicinal Plant Extracts
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초록
본 연구는 3종류의 약용식물(당귀, 목통, 골담초)을 물과 70% 에탄올로 추출하여 전자공여능과 아질산염 소거능을 조사하여 건강 식품의 제조에 필요한 기초자료를 얻고자 하였다. 3종류의 한약재중 물추출물의 수율은 목통(35.63%)>골담초(31.36%)>당귀(8.98%)의 순이었으며 에탄올 추출물의 수율은 목통(31.82%)>당귀(14.15%), 골담초(5.20%)의 순으로서 목통이 가장 높은 수율을 나타내었다. 한약재 추출물의 전자공여능은 물추출물 300-1,000 ppm에서 당귀가 62-88%로 가장 높았으며, 에탄올 추출물 300- 1,000 ppm에서는 목통이 72-87%로 가장 높은 전자공여능을 나타내었다. 아질산염 소거능은 pH 1.2에서 가장 높았고 pH가 증가할수록 감소하였다. pH 1.2에서 아질산염 소거능은 물추출물 1,000 ppm에서 35-40%로 골담초가 가장 높았고, 에탄올 추출물 1,000 ppm에서 33-42%로서 목통이 가장 높았다. 3종류의 한약재 중에서 목통은 추출물의 수율이 가장 높고 전자공여능과 아질산염 소거능이 가장 우수하여 식품에 항산화 및 아질산염 소거의 목적으로 첨가할 수 있는 적합한 약재로 생각된다.
Abstract
The purpose of this study was to investigate antioxidative and nitrite scavenging abilities of extracts of medicinal plants. Three kinds of medicinal plants, Angelica acutiloba Kitagawa (AA), Akebia quinate Decaisne (AQ), and Caragana chamlagu (CC), were extracted with water and 70% ethanol. The yields of water extracts were increased in the order of Akebia quinate Decaisne (35.63%), Caragana chamlagu (31.36%) and Angelica acutiloba (8.98%). Those of ethanol extracts were Akebia quinate Decaisne (31.82%), Angelica acutiloba Kitagawa (14.15%), and Caragana chamlagu (5.20%). The highest electron donating ability (EDA) at 300-1,000 ppm of water extract was Angelica acutiloba Kitagawa, ranged from 62% to 88% and that of ethanol extract was Akebia quinate Decaisne, ranged from 72% to 87%. Nitrite scavenging ability (NSA) of extracts measured at various pH (1.2, 3.0, 4.2, 6.0) showed highest ability in all extracts at pH 1.2 and decreased with increasing pH. The highest NSA of water extracts of 1,000 ppm at pH 1.2 showed 35-40%, in Caragana chamlagu and that of ethanol extract was 33-42%, in Akebia quinate Decaisne. From these results, Akebia quinate Decaisne was found as a potential raw material haring functionality in the respects of extract yield, EDA and NSA.
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