산삼배양근 추출물의 항산화 특성
Antioxidant Properties of Cultured Wild Ginseng Roots Extracts
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초록
산삼배양근을 식품소재 첨가물로의 이용성을 알아보고자 열수 및 70% 에탄올 추출물에 대한 항산화 활성을 측정하였다. 열수추출물의 수율은 건물당 27.86%이었으며, 70% 에탄올추출물은 18.33%이었다. 총 폴리페놀 함량은 열수 및 70% 에탄올 추출물에서 각각 17.90 ㎎/g 및 22.63 ㎎/g 이었다. Ginsenoside Rb1 및 Rg1의 함량은 각각 1.45 ㎎/g 및 0.96 mg/g 이었다. 1~20 ㎎/㎖ 농도에서 물추출물 및 70% 에탄올 추출물의 전자공여능은 각각 2.82~60.58% 및 3.88~70.88% 이었으며, 환원력에서는 70% 에탄올 추출물이 0.07~1.90이었고 물 추출물은 대체적으로 낮았다. SOD 유사활성은 물 추출물은 큰 차이가 없었으나 70% 에탄올 추출물에서는 농도가 증가함에 따라 유의적으로 증가하였다. 아질산염 소거능은 SOD 유사활성의 결과와 유사하였다. 지질산패 억제능은 70% 에탄올 추출물은 9.18~66.59%로 물 추출물의 4.74~24.88%에 비하여 1.9~2.8배 높은 활성을 나타내었다. 이상의 결과로 볼 때 산삼배양근 추출물은 70% 에탄올 추출물의 항산화성이 우수하여 산화방지제 및 기능성 증진용 소재 활용에 효과가 있을 것으로 판단된다.
Abstract
We obtained hot-water extracts (HWE) and 70% (v/v) ethanol extracts (EE) from cultured wild ginseng roots (CWGR) and determined the saponin and total polyphenol contents, and antioxidant activities. The yields of freeze-dried powder from the HWE and EE were 27.86% and 18.33% (both w/w), respectively. The total polyphenol content of the EE (22.63 ㎎/g) was higher than that of the HWE (17.90 ㎎/g). Ginsenoside-Rb1 and -Rg1 contents of hot-air-dried CWGR were 17.90 ㎎/g and 22.63 ㎎/g, respectively. The electron-donating ability of HWE and EE were 2.82–60.58% and 3.88–70.88%, respectively, and the reducing powers (OD700) were 0.02–0.17 and 0.07–1.90, respectively, at concentrations of 1–20 ㎎/㎖. Thus, the HWE reducing power was markedly lower than that of the EE, but the SOD–like activity of the EE was significantly higher than that of the HWE. The nitrite-scavenging activities of HWE and EE were 9.25–19.18% and 11.94–53.49%, respectively, at concentrations of 1–20 ㎎/㎖. Additionally, the TBARS (Thiobarbituric acid reactive substances, % value) of the EE (1–20 ㎎/㎖) was 9.18–66.59%, thus 1.9–2.8-fold greater than that of the HWE (4.74–24.88%). In conclusion, we provide experimental evidence that extracts of CWGR may be natural antioxidants.
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