과학 고등학교 학생을 대상으로 한 DNA 재조합 기술 적용의 탐색

Abstract

The rapid developments of a biotechnology in 20 century have resulted in enormous changes of our society as well as science. We have been living in the science-based society, and a genetic engineering among the biotechnology especially has affected various areas of our life. However, science high school students, anything but general high school ones, have not had chances of performing experiments for genetic engineering. Therefore, experiments related with recombinant DNA technology, a core of genetic engineering were applied to science high school students for 5 days in their school by their teacher not a professional researcher. All experiment were generally well done and students showed a significant increase of achievements (p<0.05). After doing all experimental procedures, they showed a high interest in a genetic engineering and an usual understanding in procedures of experiments. A little understanding for the experiments may be due to absence of pre-concepts about recombinant DNA and a little experimental experience of teacher. I suggest a proper time for performing the recombinant DNA experiments is after high school students fully obtaining basic concepts of recombinant DNA. Especially, plasmid isolation, DNA electrophoresis and transformation experiments among all procedures were more effective in science motivation and they are thought to be very important and useful within experimental courses for recombinant DNA technology. Therefore, I recommend the three experiments as core courses of recombinant DNA technology.