AmpFlSTR^® NGM^(TM) PCR Amplification Kit의 유효성 확인
Internal Validation of AmpFlSTR^® NGM^(TM) PCR Amplification Kit
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초록
본 연구는 유전자분석 효율을 높이고 개인식별률을 향상시키기 위해 NGM kit을 사용하기에 앞서 SWGDAM (Scientific Working Group on DNA Analysis Methods) guideline에 의거하여 민감성, 재현성, 정밀성, 정확성, 혼합반, 오염 그리고 대조시료와 현장시료의 비교 항목에 대한 유효성검사를 수행하였다. 실험결과, 0.125 ng 이상의 DNA를 사용하였을 때 완전한 유전자형이 분석되었으며, 반복실험 시 각 유전자좌위의 allele sizing이 표준편차 0.3 이하로 나타났다. 혼합반 시험의 경우 1:7 이하의 비율에서 minor contributor의 완전한 유전자형이 분석되었으며, 유전자형이 알려진 대조시료와 입증되지 않은 현장시료의 유전자형을 비교분석한 결과 서로 일치함을 확인할 수 있었다. 따라서 NGM kit은 DNA profile 분석에 적합한 것으로 판단된다.
Abstract
This study is aimed to validate the usability of NGM kit according to SWGDAM (Scientific Working Group on DNA Analysis Methods) guideline and developmental validation study by accessing sensitivity, reproducibility, precision, accuracy, mixture, contamination and known and non-probative evidence samples. Our results showed that full profiles were obtained with 0.125 ng or more of input DNA and standard deviation of allele sizing was analyzed below 0.3. Additionally in mixture study, full profiles of the minor contributor were analyzed at a ratio of 1:7 or below, and the profiles of known sample and non-probative sample were identical. Based on these results, NGM kit as a mean of analyzing DNA profiles in forensic casework was determined to be proper in this laboratory.
참고문헌 (10)
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