AmpFl STR^® Minifiler^(TM) PCR Amplification kit의 유효성 확인
Internal Validation of the AmpFlSTR^® Minifiler^(TM) PCR Amplification kit
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초록
Multiplex STR kit을 사용하는 경우, 상태가 양호한 DNA 시료에서는 전체 DNA 프로필을 분석할 수 있으나 미세 시료나 오래된 유해처럼 적은 양이거나 손상된 DNA 시료에서는 전체 DNA 프로필 분석 성공률이 낮은 편이다. 이러한 손상된 DNA 시료에 대해서 더 많은 유전자형을 분석하려는 노력에 의해 AmpFlSTR^® Minifiler^(TM) PCR Amplification kit(Applied Biosystems)이 개발 되었다. Minifiler^(TM) kit의 유효성 확인 결과, Human DNA control 9947A의 전체 프로필은 0.063 ng까지 분석되었고 재현성을 보이며, 두 시료의 혼합에서 minor peak의 전체 프로필 분석은 1 : 3 혼합 비율까지 가능하였다. 본 연구 결과를 통해 확인된 유효성 판정 기준을 적용하면 법과학 분야에서 Minifiler^(TM) kit을 이용한 실험의 분석 및 해석 시 신뢰성 있는 결과 해석이 가능할 것으로 판단된다.
Abstract
The application of multiplex STR kit results in a full DNA profile in the majority of high-quality DNA samples. However, the increased demand to analyze limited DNA samples from a low copy number or skeletal remains increases the number of samples where only a partial DNA profile is obtained due to lowquantity DNA and/or degradation of the DNA. In an effort to increase the amount of information derived from these types of DNA samples, the AmpFlSTR^® Minifiler^(TM) PCR Amplification kit has been developed. Internal validation of the Minifiler^(TM) kit revealed no differences for the STR loci while the sensitivity study showed a reproducible DNA profile with as low as 0.063 ng of Human DNA control 9947A. The full profile of a minor peak could be detected in a 1:3 of the mixture. This article describes the internal validation performed according to the SWAGDAM revised guidelines. Based on the results obtained, guidelines for the interpretation of DNA analysis procedure and DNA STR profiles drawn up to use the Minifiler^(TM) kit in forensic casework at this laboratory.
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