Prepfiler™ Forensic DNA extraction kit을 이용한 소총과 권총에서의 LCN DNA 분석
LCN DNA Analysis from Rifles and Handguns by Using Prepfiler™ Forensic DNA Extraction Kit
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참고문헌
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초록
범죄현장에서 발견된 총기에서의 DNA 분석은 사건 해결에 중요한 단서를 제공할 수 있다. 총기시료에는 소량의 DNA가 포함될 수 있고 LCN DNA(100 pg 이하의 DNA) 검사를 통해 범인을 검거하는 경우가 보고되고 있다. 본 연구는 총기(M16, K1A, Colt45 Pistol, M29 Revolver) 부위별로 유류된 유전자를 추출 kit인 Prepfiler™ kit를 사용하여 기존의 kit과의 LCN DNA 회수율 차이를 비교하였고 획득된 유전자형을 통해 발사자 식별 가능성 여부를 확인하였다. Prepfiler™ kit (0.156 ng DNA)이 Micro kit(0.093 ng DNA)보다 DNA 회수율이 높았고 대립유전자의 검출률(대립유전자 검출률 1.1%~50.6%) 비교 시 LCN DNA 분석에 더 유용하였으며, 시료 채취 부위 중 총기 손잡이 부분에서 유전자형의 검출률(33.3%~50.6%)이 높게 확인되었다.
Abstract
Firearms a type of evidence found at crime scenes and DNA profiles from firearms can be instrumental for identify a shooter in criminal investigations. This study focused on obtaining LCN DNA(less than 100pg DNA) recovery and STR profiles from each of the firearm (M16, K1A, Colt45 Pistol, M29 Revolver) surface areas using the Prepfiler™ Forensic DNA extraction kit compared with QIAamp® DNA micro kit. LCN DNA recovery of Prepfiler™ kit (0.156 ng DNA) is better than that of Micro kit (0.093ng DNA). The extrcted DNA of Prepfiler™ kit successfully amplified 1.1%~50.6% expected alleles from firearm surface areas where no alleles were observed using the Micro kit. The Grip, one of firearms surface exhibited higher DNA yields and consequently greater numbers of alleles exhibiting genotypes.
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