성폭행 여성 피해자의 세탁된 팬티에서 Semenogelin 검출: 확정검사 필요성
Detection of Semenogelin in the Washed Underpants from Female Rape Victims: Need for Confirmatory Test
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초록
성폭행 사건의 증거물인 의류와 침구류 등에서 정액 성분이 있는지를 검사하는 것은 필수적인 사 항이다. 성폭력 사건에서 중요한 생물학적 증거물인 정액에 대해 추정검사와 확정검사는 시간과 비용을 절약하는 방법으로 DNA 분석 전에 실행된다. 그러나 두 가지 검사에서 양성 반응이 확인되었다고 하 더라도 반드시 피의자의 DNA 프로필이 검출된다는 의미는 아니다. DNA 양이 부족하거나 심하게 분해된 상태로 존재하는 경우, DNA 프로필이 검출되지 않을 수도 있다. 반면에 팬티의 정액 추정검사(acid phosphatase 시험)에서는 음성이었으나 차등용해(differential lysis) 방법으로 진행한 실험에서 여성 피해 자와 남성 피의자의 DNA 프로필이 명확하게 검출되는 경우도 확인된다. 특히 팬티와 같은 직물은 여러 조건에서 세탁한 후에도 정자가 존재한다는 선행연구 보고가 있었다. 최근 광주과학수사연구소에 의뢰된성폭행 사건 증거물인 팬티에서도 동일한 상황이 확인되었다. 여러 번 세탁된 직물에서도 정자가 관찰될 수 있으므로 확정검사로 semenogelin 검출 시험이 추가적으로 수행되어야 한다. 본 연구는 이러한 사례를 보고하고, 확정검사의 필요성에 대하여 논의하였다.
Abstract
In many sexual assault cases, bedding and clothing are essential pieces of evidence that are screened for semen stains to gather DNA from the assailant. For the presence of semen, which is generally accepted as an important biological evidence in sexual assault cases, presumptive and confirmatory tests are performed before DNA analysis in a way that saves time and expense. However, positive results from both tests do not necessarily mean that the accused’s DNA profile is detected. If the amount of DNA is generally insufficient or exists in a severely decomposed state, the DNA profile may not be detected. On the other hand, although the semen test (Acid phosphatase test) on the panties was negative, DNA profiles of female victims and the male accused were sometimes clearly detected in experiments by using the differential lysis method. This is because fabrics such as panties can retain sperm even after being washed under various conditions. Recently the same situation is sometimes observed in panties, which are evidence of a sexual assault case analyzed in the Gwangju NFS. Since sperms can be observed even in fabrics that have been washed several times, the semenogelin detection test should be additionally performed as a confirmatory test. This study reports these cases and discuss on the need for confirmatory testing.
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